VHS2/YIL135C Summary Help

Standard Name VHS2 1
Systematic Name YIL135C
Feature Type ORF, Verified
Description Regulator of septin dynamics; involved in the regulation of septin dynamics at bud neck after mitotic entry, likely by stabilizing septin structure; regulated at post-translational level by cell cycle dependent phosphorylation; likely phosphorylated by Cdc28p and dephosphorylated by Cdc14p before cytokinesis; high-copy suppressor of synthetic lethality of sis2 sit4 double mutant; VHS2 has a paralog, MLF3, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description Viable in a Hal3 Sit4 background 1
Chromosomal Location
ChrIX:96375 to 95065 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VHS2 GO evidence and references
  View Computational GO annotations for VHS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
50 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 18
  • PCA: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 11
  • Negative Genetic: 7
  • Synthetic Growth Defect: 7
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 436
Molecular Weight (Da) 47,961
Isoelectric Point (pI) 10.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:96375 to 95065 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1311 96375..95065 1994-12-10 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001397
References cited on this page View Complete Literature Guide for VHS2
1) Munoz I, et al.  (2003) Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae. Yeast 20(2):157-69
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Cassani C, et al.  (2014) Vhs2 is a novel regulator of septin dynamics in budding yeast. Cell Cycle 13(10):1590-601