VAM6/YDL077C Summary Help

Standard Name VAM6 1
Systematic Name YDL077C
Alias CVT4 2 , VPL18 3 , VPL22 4 , VPS39 3 , 5
Feature Type ORF, Verified
Description Vacuolar protein involved in vacuolar membrane fusion tethering; plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p (6, 7)
Name Description VAcuolar Morphogenesis 1
Chromosomal Location
ChrIV:320120 to 316971 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All VAM6 GO evidence and references
  View Computational GO annotations for VAM6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
Large-scale survey
338 total interaction(s) for 180 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-Western: 37
  • Co-fractionation: 1
  • Co-purification: 12
  • Reconstituted Complex: 7
  • Two-hybrid: 28

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 6
  • Negative Genetic: 170
  • Phenotypic Enhancement: 3
  • Positive Genetic: 35
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 1,049
Molecular Weight (Da) 122,880
Isoelectric Point (pI) 7.04
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:320120 to 316971 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3150 320120..316971 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002235
References cited on this page View Complete Literature Guide for VAM6
1) Nakamura N, et al.  (1997) Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membranes and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae. J Biol Chem 272(17):11344-9
2) Harding TM, et al.  (1995) Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway. J Cell Biol 131(3):591-602
3) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
5) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
6) Wurmser AE, et al.  (2000) New component of the vacuolar class C-Vps complex couples nucleotide exchange on the Ypt7 GTPase to SNARE-dependent docking and fusion. J Cell Biol 151(3):551-62
7) Oka M, et al.  (2004) Molecular cloning and functional characterization of avaB, a gene encoding Vam6p/Vps39p-like protein in Aspergillus nidulans. FEMS Microbiol Lett 232(1):113-21