VAM3/YOR106W Summary Help

Standard Name VAM3 1
Systematic Name YOR106W
Alias PTH1
Feature Type ORF, Verified
Description Syntaxin-like vacuolar t-SNARE; functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region (1, 2, 3, 4, 5)
Name Description VAcuolar Morphogenesis 1
Chromosomal Location
ChrXV:519121 to 519972 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VAM3 GO evidence and references
  View Computational GO annotations for VAM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
overexpression
Resources
437 total interaction(s) for 219 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 95
  • Co-fractionation: 15
  • Co-purification: 10
  • Far Western: 1
  • FRET: 1
  • Reconstituted Complex: 25
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 8
  • Negative Genetic: 180
  • Phenotypic Enhancement: 12
  • Positive Genetic: 48
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 11

Resources
Expression Summary
histogram
Resources
Length (a.a.) 283
Molecular Weight (Da) 32,497
Isoelectric Point (pI) 7.05
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:519121 to 519972 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..852 519121..519972 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005632
References cited on this page View Complete Literature Guide for VAM3
1) Wada Y, et al.  (1992) Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. I. Isolation and characterization of two classes of vam mutants. J Biol Chem 267(26):18665-70
2) Srivastava A and Jones EW  (1998) Pth1/Vam3p is the syntaxin homolog at the vacuolar membrane of Saccharomyces cerevisiae required for the delivery of vacuolar hydrolases. Genetics 148(1):85-98
3) Sato TK, et al.  (1998) Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking. Mol Cell Biol 18(9):5308-19
4) Darsow T, et al.  (1998) Acidic di-leucine motif essential for AP-3-dependent sorting and restriction of the functional specificity of the Vam3p vacuolar t-SNARE. J Cell Biol 142(4):913-22
5) Darsow T, et al.  (1997) A multispecificity syntaxin homologue, Vam3p, essential for autophagic and biosynthetic protein transport to the vacuole. J Cell Biol 138(3):517-29