Standard Name	VAC14 1
Systematic Name	YLR386W
Feature Type	ORF, Verified
Description	Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p (1, 2, 3)
Name Description	VACuole morphology and inheritance mutant 1

Chromosomal Location	ChrXII:893628 to 896270 | ORF Map | GBrowse

	View Computational GO annotations for VAC14
Molecular Function
Manually curated	protein binding, bridging (IDA, IMP)
Biological Process
Manually curated	phosphatidylinositol biosynthetic process (IGI, IMP) positive regulation of kinase activity (IGI, IMP)
Cellular Component
Manually curated	extrinsic to vacuolar membrane (IDA) fungal-type vacuole membrane (IDA) PAS complex (IDA, IPI)

Classical genetics
misexpression	vacuolar morphology: abnormal
null	metal resistance: decreased Fig4p-GFP distribution: abnormal Atg18p-GFP distribution: abnormal carboxypeptidase Y (Prc1p) modification: decreased resistance to bortezomib: decreased vacuolar morphology: abnormal
reduction of function	1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate accumulation: increased 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate accumulation: abnormal Fab1p-GFP distribution: abnormal vacuolar morphology: abnormal
Large-scale survey
null	cell size: increased polyphosphate accumulation: abnormal competitive fitness: decreased dessication resistance: decreased fermentative growth: decreased rate heat sensitivity: increased lipid particle morphology: abnormal Fus-Mid-GFP distribution: abnormal Pho8p (ALP) modification: decreased Cps1p (CPS) modification: decreased resistance to calcofluor white: decreased resistance to ethanol: decreased resistance to sirolimus: decreased resistance to sirolimus: increased resistance to fenpropimorph: increased resistance to wortmannin: normal resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to cadmium dichloride: decreased resistance to monensin: decreased resistance to benomyl: decreased resistance to caffeine: decreased resistance to bleomycin: decreased resistance to fluconazole: decreased resistance to sodium selenide: decreased resistance to tunicamycin: decreased resistance to methyl methanesulfonate: decreased resistance to L-1,4-dithiothreitol: decreased resistance to hygromycin B: decreased stress resistance: decreased utilization of iron source: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	276 total interaction(s) for 178 unique genes/features.
Physical Interactions	Affinity Capture-MS: 18 Affinity Capture-Western: 10 PCA: 1 Reconstituted Complex: 3 Two-hybrid: 22
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 146 Phenotypic Suppression: 1 Positive Genetic: 39 Synthetic Growth Defect: 25 Synthetic Lethality: 10
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:893628 to 896270 | ORF Map | GBrowse
Last Update	Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2643 893628..896270 2004-02-05 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004378