VAC14/YLR386W Summary Help

Standard Name VAC14 1
Systematic Name YLR386W
Feature Type ORF, Verified
Description Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function (1, 2, 3, 4)
Name Description VACuole morphology and inheritance mutant 1
Chromosomal Location
ChrXII:893628 to 896270 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VAC14 GO evidence and references
  View Computational GO annotations for VAC14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Resources
Classical genetics
misexpression
null
reduction of function
Large-scale survey
null
Resources
281 total interaction(s) for 176 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-Western: 10
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 22

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 148
  • Phenotypic Suppression: 1
  • Positive Genetic: 39
  • Synthetic Growth Defect: 25
  • Synthetic Lethality: 10

Resources
Expression Summary
histogram
Resources
Length (a.a.) 880
Molecular Weight (Da) 99,771
Isoelectric Point (pI) 5.24
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:893628 to 896270 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2643 893628..896270 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004378
References cited on this page View Complete Literature Guide for VAC14
1) Bonangelino CJ, et al.  (2002) Osmotic stress-induced increase of phosphatidylinositol 3,5-bisphosphate requires Vac14p, an activator of the lipid kinase Fab1p. J Cell Biol 156(6):1015-28
2) Dove SK, et al.  (2002) Vac14 controls PtdIns(3,5)P(2) synthesis and Fab1-dependent protein trafficking to the multivesicular body. Curr Biol 12(11):885-93
3) Rudge SA, et al.  (2004) Vacuole size control: regulation of PtdIns(3,5)P2 levels by the vacuole-associated Vac14-Fig4 complex, a PtdIns(3,5)P2-specific phosphatase. Mol Biol Cell 15(1):24-36
4) Alghamdi TA, et al.  (2013) Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function. J Biol Chem 288(13):9363-72