USO1/YDL058W Summary Help

Standard Name USO1 1
Systematic Name YDL058W
Alias INT1
Feature Type ORF, Verified
Description Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex (1, 2, 3, 4)
Name Description yUSOu - transport in Japanese 1
Chromosomal Location
ChrIV:345665 to 351037 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All USO1 GO evidence and references
  View Computational GO annotations for USO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
overexpression
reduction of function
Resources
109 total interaction(s) for 86 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 16
  • Negative Genetic: 51
  • Positive Genetic: 3
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,790
Molecular Weight (Da) 206,450
Isoelectric Point (pI) 4.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:345665 to 351037 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..5373 345665..351037 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002216
References cited on this page View Complete Literature Guide for USO1
1) Nakajima H, et al.  (1991) A cytoskeleton-related gene, uso1, is required for intracellular protein transport in Saccharomyces cerevisiae. J Cell Biol 113(2):245-60
2) Cao X, et al.  (1998) Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J 17(8):2156-65
3) Sapperstein SK, et al.  (1996) Assembly of the ER to Golgi SNARE complex requires Uso1p. J Cell Biol 132(5):755-67
4) Noda Y, et al.  (2007) Specific membrane recruitment of Uso1 protein, the essential endoplasmic reticulum-to-Golgi tethering factor in yeast vesicular transport. J Cell Biochem 101(3):686-94