USA1/YML029W Summary Help

Standard Name USA1 1
Systematic Name YML029W
Feature Type ORF, Verified
Description Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p (1, 2, 3)
Name Description U1-Snp1 Associating 1
Chromosomal Location
ChrXIII:217362 to 219878 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All USA1 GO evidence and references
  View Computational GO annotations for USA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
136 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 13
  • Two-hybrid: 18

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 17
  • Phenotypic Enhancement: 29
  • Phenotypic Suppression: 12
  • Positive Genetic: 14
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 838
Molecular Weight (Da) 96,652
Isoelectric Point (pI) 4.85
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:217362 to 219878 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2517 217362..219878 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004491
References cited on this page View Complete Literature Guide for USA1
1) Awasthi S, et al.  (2001) New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem 276(33):31004-15
2) Carvalho P, et al.  (2006) Distinct ubiquitin-ligase complexes define convergent pathways for the degradation of ER proteins. Cell 126(2):361-73
3) Horn SC, et al.  (2009) Usa1 functions as a scaffold of the HRD-ubiquitin ligase. Mol Cell 36(5):782-93