URK1/YNR012W Summary

Standard Name	URK1 1
Systematic Name	YNR012W
Feature Type	ORF, Verified
Description	Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP (2, 3, 4 and see Summary Paragraph)
Name Description	URidine Kinase 1

Chromosomal Location	ChrXIV:647432 to 648937 \| ORF Map \| GBrowse

Gene Ontology Annotations All URK1 GO evidence and references

	View Computational GO annotations for URK1
Molecular Function
Manually curated	uridine kinase activity (IMP)
Biological Process
Manually curated	pyrimidine-containing compound salvage (IGI, IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	salvage pathways of pyrimidine deoxyribonucleotides salvage pathways of pyrimidine ribonucleotides

Mutant phenotypes All URK1 Phenotype evidence and references

Classical genetics
null	nutrient utilization: absent
Large-scale survey
null	competitive fitness: increased stress resistance: increased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All URK1 Interaction evidence and references

	28 total interaction(s) for 21 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 2 Two-hybrid: 12
Genetic Interactions	Negative Genetic: 5 Phenotypic Enhancement: 1 Positive Genetic: 4 Synthetic Growth Defect: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All URK1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:647432 to 648937 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1506	647432..648937	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005295

SUMMARY PARAGRAPH for URK1

Urk1p is a uridine kinase (EC 2.7.1.48) that phosphorylates uridine into UMP and cytidine into CMP in the pyrimidine ribonucleotide salvage pathway (1, 2). Urk1p can also phosphorylate deoxycytidine into dCMP in pyrimidine deoxyribonucleotide salvage (1, 2). These two pathways provide pyrimidines required for nucleic acid synthesis, amino acid synthesis, and energy (2).

URK1 is repressed in response to lithium chloride during growth on galactose (5). urk1 null mutants grow well on uridine and cytosine, but are unable to utilize cytidine, and are also resistant to 5-fluorocytidine (5-FCR) and 5-fluorouridine (5-FUR) (2, 4). urh1 urk1 double mutants are unable to utilize uridine as the sole source of pyrimidines (3).

Urk1p displays similarity to the rat myocardial uridine kinase (2).

Last updated: 2005-11-29

References cited on this page View Complete Literature Guide for URK1

1)	Kern L (1990) The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase. Nucleic Acids Res 18(17):5279
2)	Kurtz JE, et al. (1999) New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism. Curr Genet 36(3):130-6
3)	Mitterbauer R, et al. (2002) Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase. Appl Environ Microbiol 68(3):1336-43
4)	Kurtz JE, et al. (2002) The URH1 uridine ribohydrolase of Saccharomyces cerevisiae. Curr Genet 41(3):132-41
5)	Mitterbauer R, et al. (2003) A sensitive and inexpensive yeast bioassay for the mycotoxin zearalenone and other compounds with estrogenic activity. Appl Environ Microbiol 69(2):805-11