URA6/YKL024C Summary

Standard Name	URA6
Systematic Name	YKL024C
Alias	SOC8
Feature Type	ORF, Verified
Description	Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) (1, 2, 3, 4 and see Summary Paragraph)
Name Description	URAcil requiring 4, 5

Chromosomal Location	ChrXI:393140 to 392526 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 18 cM

Gene Ontology Annotations All URA6 GO evidence and references

	View Computational GO annotations for URA6
Molecular Function
Manually curated	adenylate kinase activity (IDA, IGI) uridylate kinase activity (IDA, IMP)
Biological Process
Manually curated	'de novo' pyrimidine nucleobase biosynthetic process (IDA) nucleobase-containing compound metabolic process (IDA)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA)

Pathways	de novo biosynthesis of pyrimidine ribonucleotides superpathway of histidine, purine, and pyrimidine biosynthesis

Mutant phenotypes All URA6 Phenotype evidence and references

Large-scale survey
conditional	chromosome/plasmid maintenance: abnormal
null	inviable resistance to sorbate: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All URA6 Interaction evidence and references

	15 total interaction(s) for 15 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 PCA: 2
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 9
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All URA6 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXI:393140 to 392526 | |

Note: this feature is encoded on the Crick strand.

: 18 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..615	393140..392526	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001507

SUMMARY PARAGRAPH for URA6

URA6 encodes uridine-monophosphate kinase, an enzyme involved in pyrimidine biosynthesis (4). The structure and mechanism of nucleoside-monophosphate kinases has been extensively reviewed in (6). The URA6 gene was first isolated as a uracil auxotroph (4). In addition, URA6 was independently isolated as SOC8, a partial suppressor of cdc8 mutations (3, 7). CDC8 encodes a thymidylate kinase; when overexpressed, Ura6p can provide enough thymidylate kinase activity to compensate for the loss of Cdc8p (3, 7). Ura6p can also phosphorylate other nucleosides, but not as efficiently as UMP (8, 9, 2).

Last updated: 2000-01-20

References cited on this page View Complete Literature Guide for URA6

1)	Jong A, et al. (1993) Characteristics, substrate analysis, and intracellular location of Saccharomyces cerevisiae UMP kinase. Arch Biochem Biophys 304(1):197-204
2)	Ma JJ, et al. (1990) Purification and characterization of Saccharomyces cerevisiae uridine monophosphate kinase. J Biol Chem 265(31):19122-7
3)	Choi WJ, et al. (1989) The Saccharomyces cerevisiae SOC8-1 gene and its relationship to a nucleotide kinase. J Biol Chem 264(26):15593-9
4)	Liljelund P and Lacroute F (1986) Genetic characterization and isolation of the Saccharomyces cerevisiae gene coding for uridine monophosphokinase. Mol Gen Genet 205(1):74-81
5)	Liljelund P, et al. (1989) Primary structure of the S. cerevisiae gene encoding uridine monophosphokinase. Biochem Biophys Res Commun 165(1):464-73
6)	Yan H and Tsai MD (1999) Nucleoside monophosphate kinases: structure, mechanism, and substrate specificity. Adv Enzymol Relat Areas Mol Biol 73:103-34, x
7)	Jiang ZR, et al. (1991) Molecular characterization of Saccharomyces cerevisiae URA6 gene. DNA sequence, mutagenesis analysis, and cell cycle regulation relevant to its suppression mechanism to cdc8 mutation. J Biol Chem 266(27):18287-93
8)	Hodges PE, et al. (1999) The Yeast Proteome Database (YPD): a model for the organization and presentation of genome-wide functional data. Nucleic Acids Res 27(1):69-73
9)	Schricker R, et al. (1992) The adenylate kinase family in yeast: identification of URA6 as a multicopy suppressor of deficiency in major AMP kinase. Gene 122(1):111-8