URA6/YKL024C Summary Help

Standard Name URA6 1
Systematic Name YKL024C
Alias SOC8
Feature Type ORF, Verified
Description Uridylate kinase; catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) (1, 2, 3, 4 and see Summary Paragraph)
Name Description URAcil requiring 1, 5
Chromosomal Location
ChrXI:393140 to 392526 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 18 cM
Gene Ontology Annotations All URA6 GO evidence and references
  View Computational GO annotations for URA6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Large-scale survey
18 total interaction(s) for 17 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • PCA: 2

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 9

Expression Summary
Length (a.a.) 204
Molecular Weight (Da) 22,933
Isoelectric Point (pI) 6.53
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:393140 to 392526 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 18 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..615 393140..392526 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001507

URA6 encodes uridine-monophosphate kinase, an enzyme involved in pyrimidine biosynthesis (1). The structure and mechanism of nucleoside-monophosphate kinases has been extensively reviewed in (6). The URA6 gene was first isolated as a uracil auxotroph (1). In addition, URA6 was independently isolated as SOC8, a partial suppressor of cdc8 mutations (4, 7). CDC8 encodes a thymidylate kinase; when overexpressed, Ura6p can provide enough thymidylate kinase activity to compensate for the loss of Cdc8p (4, 7). Ura6p can also phosphorylate other nucleosides, but not as efficiently as UMP (8, 9, 3).

Last updated: 2000-01-20 Contact SGD

References cited on this page View Complete Literature Guide for URA6
1) Liljelund P and Lacroute F  (1986) Genetic characterization and isolation of the Saccharomyces cerevisiae gene coding for uridine monophosphokinase. Mol Gen Genet 205(1):74-81
2) Jong A, et al.  (1993) Characteristics, substrate analysis, and intracellular location of Saccharomyces cerevisiae UMP kinase. Arch Biochem Biophys 304(1):197-204
3) Ma JJ, et al.  (1990) Purification and characterization of Saccharomyces cerevisiae uridine monophosphate kinase. J Biol Chem 265(31):19122-7
4) Choi WJ, et al.  (1989) The Saccharomyces cerevisiae SOC8-1 gene and its relationship to a nucleotide kinase. J Biol Chem 264(26):15593-9
5) Liljelund P, et al.  (1989) Primary structure of the S. cerevisiae gene encoding uridine monophosphokinase. Biochem Biophys Res Commun 165(1):464-73
6) Yan H and Tsai MD  (1999) Nucleoside monophosphate kinases: structure, mechanism, and substrate specificity. Adv Enzymol Relat Areas Mol Biol 73:103-34, x
7) Jiang ZR, et al.  (1991) Molecular characterization of Saccharomyces cerevisiae URA6 gene. DNA sequence, mutagenesis analysis, and cell cycle regulation relevant to its suppression mechanism to cdc8 mutation. J Biol Chem 266(27):18287-93
8) Hodges PE, et al.  (1999) The Yeast Proteome Database (YPD): a model for the organization and presentation of genome-wide functional data. Nucleic Acids Res 27(1):69-73
9) Schricker R, et al.  (1992) The adenylate kinase family in yeast: identification of URA6 as a multicopy suppressor of deficiency in major AMP kinase. Gene 122(1):111-8