URA1/YKL216W Summary Help

Standard Name URA1 1
Systematic Name YKL216W
Feature Type ORF, Verified
Description Dihydroorotate dehydrogenase; catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid (2, 3, 4 and see Summary Paragraph)
Name Description URAcil requiring 2
Chromosomal Location
ChrXI:25215 to 26159 | ORF Map | GBrowse
Gbrowse
Genetic position: -159.7 cM
Gene Ontology Annotations All URA1 GO evidence and references
  View Computational GO annotations for URA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 19 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
54 total interaction(s) for 49 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 3
  • PCA: 2

Genetic Interactions
  • Negative Genetic: 26
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 314
Molecular Weight (Da) 34,801
Isoelectric Point (pI) 5.92
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:25215 to 26159 | ORF Map | GBrowse
SGD ORF map
Genetic position: -159.7 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..945 25215..26159 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001699
SUMMARY PARAGRAPH for URA1

URA1 encodes dihydroorotic acid dehydrogenase (DHOdehase), an enzyme involved in the de novo synthesis of pyrimidine ribonucleotides (shown here; 5). Ura1p is responsible for catalyzing the fourth step in this pathway, the conversion of dihydroorotic acid to orotic acid (5). Unlike Schizosaccharomyces pombe and mammalian DHOdehase, which localizes to the mitochondria, S. cerevisiae DHOdehase is cytoplasmic (6, reviewed in 7). Loss of Ura1p activity leads to a lack of cell growth unless uracil or uridine is added to the media (reviewed in 7).

Uracil starvation or increased levels of the pyrimidine biosynthesis pathway intermediate dihydoorotic acid (DHO) can induce URA1 expression 6-8 fold (3). This regulation is mediated by the transcriptional activator Ppr1p, which binds to the UASURA site (CGGN6CCG) in the promoters of URA1, URA3, and URA4 (8). DNA-bound Ppr1p is transcriptionally inactive, but the addition of DHO converts Ppr1p to an active state that interacts with RNA polymerase II, leading to increased expression of the URA genes (8). URA1 expression has been shown to be downregulated by DMSO and also by lithium, a compound that is toxic to yeast cells grown in galactose (9, 10).

Last updated: 2005-12-15 Contact SGD

References cited on this page View Complete Literature Guide for URA1
1) Link, A. and Olson, M.  (1989) Personal Communication, Mortimer Map Edition 10
2) Guerry-Kopecko P and Wickner RB  (1980) Cloning of the URA1 gene of Saccharomyces cerevisiae. J Bacteriol 143(3):1530-3
3) Lacroute F  (1968) Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol 95(3):824-32
4) Vorisek J, et al.  (2002) Enzymatic activities of Ura2 and Ura1 proteins (aspartate carbamoyltransferase and dihydro-orotate dehydrogenase) are present in both isolated membranes and cytoplasm of Saccharomyces cerevisiae. Yeast 19(5):449-57
5) Roy A  (1992) Nucleotide sequence of the URA1 gene of Saccharomyces cerevisiae. Gene 118(1):149-50
6) Nagy M, et al.  (1992) Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts. Proc Natl Acad Sci U S A 89(19):8966-70
7) Jones ME  (1992) Orotidylate decarboxylase of yeast and man. Curr Top Cell Regul 33():331-42
8) Flynn PJ and Reece RJ  (1999) Activation of transcription by metabolic intermediates of the pyrimidine biosynthetic pathway. Mol Cell Biol 19(1):882-8
9) Bro C, et al.  (2003) Transcriptional, proteomic, and metabolic responses to lithium in galactose-grown yeast cells. J Biol Chem 278(34):32141-9
10) Zhang W, et al.  (2003) Microarray analyses of the metabolic responses of Saccharomyces cerevisiae to organic solvent dimethyl sulfoxide. J Ind Microbiol Biotechnol 30(1):57-69