UGP1/YKL035W Summary Help

Standard Name UGP1 1
Systematic Name YKL035W
Feature Type ORF, Verified
Description UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication (1, 2, 3, 4 and see Summary Paragraph)
Name Description UDP-glucose pyrophosphorylase 1
Chromosomal Location
ChrXI:369891 to 371390 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All UGP1 GO evidence and references
  View Computational GO annotations for UGP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 21 genes
Resources
Pathways
Classical genetics
conditional
null
overexpression
reduction of function
repressible
Large-scale survey
conditional
null
reduction of function
unspecified
Resources
72 total interaction(s) for 60 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 3
  • Co-crystal Structure: 1
  • Co-purification: 1
  • PCA: 1
  • Protein-peptide: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 4
  • Negative Genetic: 17
  • Positive Genetic: 7
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 499
Molecular Weight (Da) 55,987
Isoelectric Point (pI) 7.46
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:369891 to 371390 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1500 369891..371390 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001518
SUMMARY PARAGRAPH for UGP1

Ugp1p is a UDP-glucose pyrophosphorylase (EC:2.7.7.9) which catalyzes the reversible formation of UDP-glucose from glucose 1-phosphate and UTP (1, 5). UDP-glucose serves as a glucosyl donor in several metabolic pathways including the biosynthesis of glycogen and trehalose, the formation of cell wall beta-glucans and glucomannoproteins, protein N-glycosylation, and galactose entry into glycolysis (2).

Expression of UGP1 appears to be inhibited by Pho85p through Pho4p (3). Overexpression during growth on galactose results in slower growth, increased sensitivity to calcofluor white, and increased protein glycosylation, as compared to wild type (1). A ten-fold reduction in Ugp1p activity results in a multi-budding phenotype, increased resistance to zymolyase, decreased glycogen and cell wall beta-glucan levels, and increased sensitivity to cell wall interfering drugs (1, 2). Ugp1 null mutants are inviable.

UDP-glucose pyrophosphorylase is conserved across many species, and yeast Ugp1p is similar to orthologs in cow, human, and potato tuber (1). Yeast Ugp1p complements the defects of an E. coli mutant strain defective for UDP-glucose pyrophosphorylase (galU) (1). Overexpression of either Ugp1p or human UDP-glucose pyrophosphorylase (hUGP2) complements the growth defects of a gal7 null mutant on galactose, suggesting that increased expression of hUGP2 in humans could alleviate poor outcomes in patients with classic galactosemia (5).

Last updated: 2005-09-13 Contact SGD

References cited on this page View Complete Literature Guide for UGP1
1) Daran JM, et al.  (1995) Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae. Eur J Biochem 233(2):520-30
2) Daran JM, et al.  (1997) Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae. FEMS Microbiol Lett 153(1):89-96
3) Nishizawa M, et al.  (2001) Pho85 kinase, a yeast cyclin-dependent kinase, regulates the expression of UGP1 encoding UDP-glucose pyrophosphorylase. Yeast 18(3):239-49
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Lai K and Elsas LJ  (2000) Overexpression of human UDP-glucose pyrophosphorylase rescues galactose-1-phosphate uridyltransferase-deficient yeast. Biochem Biophys Res Commun 271(2):392-400