UGA4/YDL210W Summary Help

Standard Name UGA4 1, 2
Systematic Name YDL210W
Feature Type ORF, Verified
Description GABA (gamma-aminobutyrate) permease; serves as a GABA transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane (1, 3, 4, 5 and see Summary Paragraph)
Name Description Utilization of GAba 6
Chromosomal Location
ChrIV:84270 to 85985 | ORF Map | GBrowse
Gene Ontology Annotations All UGA4 GO evidence and references
  View Computational GO annotations for UGA4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Large-scale survey
37 total interaction(s) for 36 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 3
  • PCA: 8
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Negative Genetic: 12
  • Phenotypic Suppression: 1
  • Positive Genetic: 6

Expression Summary
Length (a.a.) 571
Molecular Weight (Da) 61,872
Isoelectric Point (pI) 6.61
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:84270 to 85985 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1716 84270..85985 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000002369

UGA4 encodes a vacuolar membrane localized permease involved in the transport and utilization of the nitrogen source gamma-aminobutyric acid (GABA) (1, 5, 2). GABA uptake is also mediated by the general amino acid permease, Gap1p and the proline-specific permease, Put4p (1, 2). Triple mutants grow poorly when GABA is the sole nitrogen source, and have a very low rate of GABA uptake under all growth conditions tested (1, 2). Uga4p also catalyzes the transport of the polyamine putrescine, and delta-aminolevulinic acid (ALA), a precursor of porphyrin biosynthesis (7, 5). Based on both functional and phylogenetic criteria, Uga4p has been classified as a member of the amino acid/choline transporter (ACT) subfamily, TC 2.A.3.4, (8, 9), and by sequence similarity is most closely related to HNM1, the yeast choline transporter (1). Although UGA4 shares limited sequence similarity to previously identified GABA transporters in other species (1), functional complementation of the triple mutant (uga4 gap1 put4) has been used to identify GABA transporters from Candida albicans (GPT1) (10) and Arabidopsis thaliana (AtGAT1) (11).

The expression of UGA4 is induced by GABA, as is that of UGA1 and UGA2, genes encoding enzymes that catalyze the two-step conversion of GABA to succinate, through the actions of Uga3p, a pathway specific transcriptional activator, and Dal81p, a pleiotrophic activator that regulates multiple nitrogen catabolic genes (4, 12, 1, 13). These factors bind to an upstream activation site (UAS), called the UAS-GABA element, located in the promoters of the UGA genes (14, 15). When cells are grown in the presence of a poor nitrogen source that lacks GABA, the expression of UGA4 is negatively regulated by the transcriptional repressor Dal80p, a member of the GATA family of sequence-specific DNA binding proteins that binds to adjacent repeats of a distinct upstream element (UAS-GATA) and functions as a conditional transcriptional repressor (4, 12, 16, 17, 18, 15). Upon the addition of GABA, the transcriptional activator Gln3p, a second GATA family member, competes with Dal80p for UAS-GATA sites, resulting in increased UGA4 expression (19, 20, 21). UGA4 is also subject to nitrogen catabolite repression (NCR) (18, 17, 15, 19). When cells are grown in the presence of a rich nitrogen source, Ure2p, a transcriptional corepressor, binds to Gln3p and keeps it in an inactive state by cytoplasmic sequestration (22). Finally, UGA4 is regulated by carbon source (23), and also by the extracellular amino acid concentration, through the actions of the SPS plasma membrane amino acid sensor complex (Ssy1p, Ptr3p and Ssy5p) (24).

Last updated: 2008-02-13 Contact SGD

References cited on this page View Complete Literature Guide for UGA4
1) Andre B, et al.  (1993) Cloning and expression of the UGA4 gene coding for the inducible GABA-specific transport protein of Saccharomyces cerevisiae. Mol Gen Genet 237(1-2):17-25
2) Grenson M, et al.  (1987) 4-Aminobutyric acid (GABA) uptake in Baker's yeast Saccharomyces cerevisiae is mediated by the general amino acid permease, the proline permease and a GABA specific permease integrated into the GABA-catabolic pathway. Life Sci Adv Biochem 6:35-39
3) Correa Garcia S, et al.  (1997) Carbon and nitrogen sources regulate delta-aminolevulinic acid and gamma-aminobutyric acid transport in Saccharomyces cerevisiae. Int J Biochem Cell Biol 29(8-9):1097-101
4) Vissers S, et al.  (1989) Positive and negative regulatory elements control the expression of the UGA4 gene coding for the inducible 4-aminobutyric-acid-specific permease in Saccharomyces cerevisiae. Eur J Biochem 181(2):357-61
5) Uemura T, et al.  (2004) Uptake of GABA and putrescine by UGA4 on the vacuolar membrane in Saccharomyces cerevisiae. Biochem Biophys Res Commun 315(4):1082-7
6) Ramos F, et al.  (1985) Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae. Eur J Biochem 149(2):401-4
7) Bermudez Moretti M, et al.  (1996) delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4. Cell Mol Biol (Noisy-le-grand) 42(4):519-23
8) Saier MH Jr  (2000) A functional-phylogenetic classification system for transmembrane solute transporters. Microbiol Mol Biol Rev 64(2):354-411
9) De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70
10) McNemar MD, et al.  (2001) Isolation of a gene encoding a putative polyamine transporter from Candida albicans, GPT1. Yeast 18(6):555-61
11) Meyer A, et al.  (2006) AtGAT1, a high affinity transporter for gamma-aminobutyric acid in Arabidopsis thaliana. J Biol Chem 281(11):7197-204
12) Vissers S, et al.  (1990) Induction of the 4-aminobutyrate and urea-catabolic pathways in Saccharomyces cerevisiae. Specific and common transcriptional regulators. Eur J Biochem 187(3):611-6
13) Bricmont PA, et al.  (1991) The DAL81 gene product is required for induced expression of two differently regulated nitrogen catabolic genes in Saccharomyces cerevisiae. Mol Cell Biol 11(2):1161-6
14) Idicula AM, et al.  (2002) Binding and activation by the zinc cluster transcription factors of Saccharomyces cerevisiae. Redefining the UASGABA and its interaction with Uga3p. J Biol Chem 277(48):45977-83
15) Talibi D, et al.  (1995) Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Nucleic Acids Res 23(4):550-7
16) Cunningham TS and Cooper TG  (1993) The Saccharomyces cerevisiae DAL80 repressor protein binds to multiple copies of GATAA-containing sequences (URSGATA). J Bacteriol 175(18):5851-61
17) Cunningham TS, et al.  (1994) The UGA4 UASNTR site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae. J Bacteriol 176(15):4718-25
18) Andre B, et al.  (1995) Two mutually exclusive regulatory systems inhibit UASGATA, a cluster of 5'-GAT(A/T)A-3' upstream from the UGA4 gene of Saccharomyces cerevisiae. Nucleic Acids Res 23(4):558-64
19) Cunningham TS, et al.  (1996) G1n3p is capable of binding to UAS(NTR) elements and activating transcription in Saccharomyces cerevisiae. J Bacteriol 178(12):3470-9
20) Coffman JA, et al.  (1997) Cross regulation of four GATA factors that control nitrogen catabolic gene expression in Saccharomyces cerevisiae. J Bacteriol 179(11):3416-29
21) Soussi-Boudekou S, et al.  (1997) Gzf3p, a fourth GATA factor involved in nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol Microbiol 23(6):1157-68
22) Kulkarni AA, et al.  (2001) Gln3p nuclear localization and interaction with Ure2p in Saccharomyces cerevisiae. J Biol Chem 276(34):32136-44
23) Luzzani C, et al.  (2007) New insights into the regulation of the Saccharomyces cerevisiae UGA4 gene: two parallel pathways participate in carbon-regulated transcription. Microbiology 153(Pt 11):3677-3684
24) Bermudez Moretti M, et al.  (2005) Expression of the UGA4 gene encoding the delta-aminolevulinic and gamma-aminobutyric acids permease in Saccharomyces cerevisiae is controlled by amino acid-sensing systems. Arch Microbiol 184(2):137-40