UFD1/YGR048W Summary Help

Standard Name UFD1 1
Systematic Name YGR048W
Feature Type ORF, Verified
Description Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) (2, 3, 4, 5, 6, 7, 8)
Name Description Ubiquitin Fusion Degradation protein 1
Chromosomal Location
ChrVII:589826 to 590911 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All UFD1 GO evidence and references
  View Computational GO annotations for UFD1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Resources
Classical genetics
conditional
null
overexpression
Large-scale survey
conditional
null
reduction of function
repressible
Resources
131 total interaction(s) for 78 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-Western: 31
  • Co-purification: 2
  • PCA: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 16

Genetic Interactions
  • Negative Genetic: 36
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 361
Molecular Weight (Da) 39,810
Isoelectric Point (pI) 6.05
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:589826 to 590911 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1086 589826..590911 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003280
References cited on this page View Complete Literature Guide for UFD1
1) Johnson ES, et al.  (1995) A proteolytic pathway that recognizes ubiquitin as a degradation signal. J Biol Chem 270(29):17442-56
2) Hitchcock AL, et al.  (2001) The conserved npl4 protein complex mediates proteasome-dependent membrane-bound transcription factor activation. Mol Biol Cell 12(10):3226-41
3) Ye Y, et al.  (2001) The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol. Nature 414(6864):652-6
4) Braun S, et al.  (2002) Role of the ubiquitin-selective CDC48(UFD1/NPL4 )chaperone (segregase) in ERAD of OLE1 and other substrates. EMBO J 21(4):615-21
5) Bays NW, et al.  (2001) HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins. Mol Biol Cell 12(12):4114-28
6) Rape M, et al.  (2001) Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone. Cell 107(5):667-77
7) Park S, et al.  (2005) Ufd1 exhibits the AAA-ATPase fold with two distinct ubiquitin interaction sites. Structure 13(7):995-1005
8) Barbin L, et al.  (2010) The Cdc48-Ufd1-Npl4 complex is central in ubiquitin-proteasome triggered catabolite degradation of fructose-1,6-bisphosphatase. Biochem Biophys Res Commun 394(2):335-41