UBX2/YML013W Summary Help

Standard Name UBX2 1
Systematic Name YML013W
Alias SEL1 2
Feature Type ORF, Verified
Description Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis (1, 2, 3, 4, 5)
Name Description UBiquitin regulatory X 1
Chromosomal Location
ChrXIII:244149 to 245903 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All UBX2 GO evidence and references
  View Computational GO annotations for UBX2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
385 total interaction(s) for 209 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 33
  • Affinity Capture-Western: 27
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 6
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 178
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 71
  • Positive Genetic: 45
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 584
Molecular Weight (Da) 66,761
Isoelectric Point (pI) 5.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:244149 to 245903 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1755 244149..245903 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004475
References cited on this page View Complete Literature Guide for UBX2
1) Schuberth C, et al.  (2004) Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation. EMBO Rep 5(8):818-24
2) Bartkeviciute D and Sasnauskas K  (2003) Studies of yeast Kluyveromyces lactis mutations conferring super-secretion of recombinant proteins. Yeast 20(1):1-11
3) Schuberth C and Buchberger A  (2005) Membrane-bound Ubx2 recruits Cdc48 to ubiquitin ligases and their substrates to ensure efficient ER-associated protein degradation. Nat Cell Biol 7(10):999-1006
4) Neuber O, et al.  (2005) Ubx2 links the Cdc48 complex to ER-associated protein degradation. Nat Cell Biol 7(10):993-8
5) Wang CW and Lee SC  (2012) The ubiquitin-like (UBX)-domain-containing protein Ubx2/Ubxd8 regulates lipid droplet homeostasis. J Cell Sci 125(Pt 12):2930-9