UBR1/YGR184C Summary Help

Standard Name UBR1 1
Systematic Name YGR184C
Alias PTR1 2
Feature Type ORF, Verified
Description E3 ubiquitin ligase (N-recognin); heterodimerizes with Rad6p to ubiquitinate substrates in the N-end rule pathway; role in endoplasmic reticulum-associated protein degradation (ERAD) in the absence of canonical ER membrane ligases or after stress; major role in targeting misfolded cytosolic proteins for degradation; regulates peptide transport via Cup9p ubiquitination; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) (1, 3, 4, 5, 6, 7, 8, 9)
Chromosomal Location
ChrVII:865753 to 859901 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All UBR1 GO evidence and references
  View Computational GO annotations for UBR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
237 total interaction(s) for 155 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 20
  • Biochemical Activity: 2
  • Co-purification: 2
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 112
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 9
  • Positive Genetic: 13
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 6
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,950
Molecular Weight (Da) 224,836
Isoelectric Point (pI) 5.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:865753 to 859901 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..5853 865753..859901 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003416
References cited on this page View Complete Literature Guide for UBR1
1) Bartel B, et al.  (1990) The recognition component of the N-end rule pathway. EMBO J 9(10):3179-89
2) Island MD, et al.  (1991) Isolation and characterization of S. cerevisiae mutants deficient in amino acid-inducible peptide transport. Curr Genet 20(6):457-63
3) Dohmen RJ, et al.  (1991) The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme. Proc Natl Acad Sci U S A 88(16):7351-5
4) Byrd C, et al.  (1998) The N-end rule pathway controls the import of peptides through degradation of a transcriptional repressor. EMBO J 17(1):269-77
5) Xie Y and Varshavsky A  (2000) Physical association of ubiquitin ligases and the 26S proteasome. Proc Natl Acad Sci U S A 97(6):2497-502
6) Xia Z, et al.  (2008) Substrate-binding Sites of UBR1, the Ubiquitin Ligase of the N-end Rule Pathway. J Biol Chem 283(35):24011-28
7) Zenker M, et al.  (2005) Deficiency of UBR1, a ubiquitin ligase of the N-end rule pathway, causes pancreatic dysfunction, malformations and mental retardation (Johanson-Blizzard syndrome). Nat Genet 37(12):1345-50
8) Khosrow-Khavar F, et al.  (2012) The yeast ubr1 ubiquitin ligase participates in a prominent pathway that targets cytosolic thermosensitive mutants for degradation. G3 (Bethesda) 2(5):619-28
9) Stolz A, et al.  (2013) Previously unknown role for the ubiquitin ligase Ubr1 in endoplasmic reticulum-associated protein degradation. Proc Natl Acad Sci U S A 110(38):15271-6