UBP3/YER151C Summary Help

UBP3 BASIC INFORMATION

Standard Name UBP3 1
Systematic Name YER151C
Alias BLM3 2
Feature Type ORF, Verified
Description Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde transport between endoplasmic reticulum and Golgi compartments; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin (1, 3, 4, 5 and see Summary Paragraph)
Name Description UBiquitin-specific Protease 1
GO Annotations All UBP3 GO evidence and references
    View Computational GO annotations for UBP3
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Mutant Phenotype All UBP3 Phenotype details and references
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
overexpression
Interactions UBP3 All interactions details and references
370 total interaction(s) for 269 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 7
  • Co-crystal Structure: 1
  • Co-purification: 7
  • Reconstituted Complex: 5
  • Two-hybrid: 7
Genetic Interactions
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 188
  • Phenotypic Suppression: 42
  • Synthetic Growth Defect: 58
  • Synthetic Lethality: 22
  • Synthetic Rescue: 2
Sequence Information
ChrV:472419 to 469681 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2739 472419..469681 1996-07-31 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000953

UBP3 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for UBP3

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
BLM10Nomenclature conflictThe name BLM3, originally referring to a genetic locus, has also been associated with YFL007W (now called BLM10) due to an error in cloning. It was later determined that blm3-1 is an allele of UBP3/YER151C.

SUMMARY PARAGRAPH for UBP3

BLM3 was identified genetically in a large screen looking for mutations conferring hypersensitivity to the drug bleomycin and radiation (6, 7). The ORF YFL007W was cloned as a multicopy suppressor of the codominant blm3-1 mutation (8). Complete deletions of the YFL007W ORF indicated that cells are hypersensitive to bleomycin and viable in the absence of the drug (8). Subsequent resequencing of the region around YFL007W indicated the presence of an additional G, resulting in the original YFL006W ORF being merged into YFL007W (9, 10). Genetic mapping and sequencing of the expanded YFL007W ORF in the blm3-1 mutant indicated that YFL007W was an extragenic suppressor of the blm3-1 mutation (11). Thus, YFL007W was renamed BLM10 (11). The gene corresponding to the original blm3-1 mutation was later identified as UBP3 (2).

Last updated: 2008-07-22

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for UBP3]

1) Baker RT, et al.  (1992) Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family. J Biol Chem 267(32):23364-75
2) McCullock S, et al.  (2006) blm3-1 Is an Allele of UBP3, a Ubiquitin Protease that Appears to Act During Transcription of Damaged DNA. J Mol Biol 363(3):660-72
3) Moazed D and Johnson D  (1996) A deubiquitinating enzyme interacts with SIR4 and regulates silencing in S. cerevisiae. Cell 86(4):667-77
4) Cohen M, et al.  (2003) Ubp3 requires a cofactor, Bre5, to specifically de-ubiquitinate the COPII protein, Sec23. Nat Cell Biol 5(7):661-7
5) Cohen M, et al.  (2003) Deubiquitination, a new player in Golgi to endoplasmic reticulum retrograde transport. J Biol Chem 278(52):51989-92
6) Moore CW  (1980) Isolation and partial characterization of mutants of Saccharomyces cerevisiae altered in sensitivities to lethal effects of bleomycins. J Antibiot (Tokyo) 33(11):1369-75
7) Moore CW  (1991) Further characterizations of bleomycin-sensitive (blm) mutants of Saccharomyces cerevisiae with implications for a radiomimetic model. J Bacteriol 173(11):3605-8
8) Febres DE, et al.  (2001) The novel BLM3 gene encodes a protein that protects against lethal effects of oxidative damage. Cell Mol Biol (Noisy-le-grand) 47(7):1149-62
9) Robben J, et al.  (2002) Revisiting the yeast chromosome VI DNA sequence reveals a correction merging YFL007w and YFL006w to a single ORF. Yeast 19(8):699-702
10) Kellis M, et al.  (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
11) Doherty K, et al.  (2004) Expression of the expanded YFL007w ORF and assignment of the gene name BLM10. Yeast 21(12):1021-3