UBP2/YOR124C Summary

UBP2 BASIC INFORMATION

Standard Name	UBP2 1
Systematic Name	YOR124C
Feature Type	ORF, Verified
Description	Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity (1, 2, 3)
Name Description	UBiquitin-specific Protease 1

GO Annotations	All UBP2 GO evidence and references
	View Computational GO annotations for UBP2
Molecular Function
Manually curated	ubiquitin-specific protease activity (IDA)
Biological Process
Manually curated	protein deubiquitination (IDA) ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway (IGI, IMP, IPI)
Cellular Component
Manually curated	cytoplasm (IC)

Mutant Phenotype	All UBP2 Phenotype details and references
Classical genetics
null	Fur4p-GFP distribution: abnormal Rsp5p ubiquitination substrates modification: abnormal resistance to (S)-azetidine-2-carboxylic acid: decreased resistance to 5-fluorouracil: decreased resistance to alpha factor: decreased
overexpression	vegetative growth: decreased
Large-scale survey
null	competitive fitness: increased resistance to hydroxyurea: decreased resistance to arsenite(3-): decreased resistance to rapamycin: decreased viable

Interactions	UBP2 All interactions details and references
	View additional details at BioGRID
	99 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 18 Affinity Capture-RNA: 1 Affinity Capture-Western: 7 Biochemical Activity: 5 Reconstituted Complex: 1 Two-hybrid: 6
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 50 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 2 Synthetic Rescue: 1

Sequence Information

ChrXV:558643 to 554825 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3819	558643..554825	2006-01-05	1996-07-31

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005650

UBP2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for UBP2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for UBP2]

1)	Baker RT, et al. (1992) Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family. J Biol Chem 267(32):23364-75
2)	Layfield R, et al. (1999) Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes. Anal Biochem 274(1):40-9
3)	Lam MH, et al. (2009) Interaction of the Deubiquitinating Enzyme Ubp2 and the E3 Ligase Rsp5 Is Required for Transporter/Receptor Sorting in the Multivesicular Body Pathway. PLoS ONE 4(1):e4259

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