UBP2/YOR124C Summary Help

Standard Name UBP2 1
Systematic Name YOR124C
Feature Type ORF, Verified
Description Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; deubiquitinates Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity (1, 2, 3, 4)
Name Description UBiquitin-specific Protease 1
Chromosomal Location
ChrXV:558642 to 554824 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All UBP2 GO evidence and references
  View Computational GO annotations for UBP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
122 total interaction(s) for 82 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Biochemical Activity: 8
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 51
  • Phenotypic Enhancement: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,272
Molecular Weight (Da) 146,353
Isoelectric Point (pI) 4.94
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:558642 to 554824 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3819 558642..554824 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005650
References cited on this page View Complete Literature Guide for UBP2
1) Baker RT, et al.  (1992) Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family. J Biol Chem 267(32):23364-75
2) Layfield R, et al.  (1999) Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes. Anal Biochem 274(1):40-9
3) Lam MH, et al.  (2009) Interaction of the Deubiquitinating Enzyme Ubp2 and the E3 Ligase Rsp5 Is Required for Transporter/Receptor Sorting in the Multivesicular Body Pathway. PLoS One 4(1):e4259
4) Lam MH and Emili A  (2013) Ubp2 regulates rsp5 ubiquitination activity in vivo and in vitro. PLoS One 8(9):e75372