UBI4/YLL039C Summary Help

Standard Name UBI4 1
Systematic Name YLL039C
Alias SCD2 , UB14
Feature Type ORF, Verified
Description Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Name Description Ubiquitin 1
Chromosomal Location
ChrXII:65207 to 64062 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All UBI4 GO evidence and references
  View Computational GO annotations for UBI4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 22 genes
Classical genetics
Large-scale survey
9324 total interaction(s) for 3507 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7940
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 298
  • Biochemical Activity: 25
  • Co-crystal Structure: 11
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 711
  • Far Western: 2
  • Protein-peptide: 5
  • Reconstituted Complex: 81
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Rescue: 37
  • Negative Genetic: 149
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 5
  • Positive Genetic: 10
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 4
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 381
Molecular Weight (Da) 42,826
Isoelectric Point (pI) 7.87
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:65207 to 64062 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1146 65207..64062 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003962
References cited on this page View Complete Literature Guide for UBI4
1) Ozkaynak E, et al.  (1987) The yeast ubiquitin genes: a family of natural gene fusions. EMBO J 6(5):1429-39
2) Shih SC, et al.  (2000) Monoubiquitin carries a novel internalization signal that is appended to activated receptors. EMBO J 19(2):187-98
3) Finley D, et al.  (1994) Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant. Mol Cell Biol 14(8):5501-9
4) Lucero P, et al.  (2000) Monoubiquitination is sufficient to signal internalization of the maltose transporter in Saccharomyces cerevisiae. J Bacteriol 182(1):241-3
5) Finley D, et al.  (1987) The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses. Cell 48(6):1035-46
6) Ozkaynak E, et al.  (1984) The yeast ubiquitin gene: head-to-tail repeats encoding a polyubiquitin precursor protein. Nature 312(5995):663-6
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76