UBA4/YHR111W Summary Help

Standard Name UBA4 1
Systematic Name YHR111W
Alias YHR1
Feature Type ORF, Verified
Description E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p (1, 2, 3, 4, 5)
Name Description UBiquitin-Activating 1
Chromosomal Location
ChrVIII:333072 to 334394 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All UBA4 GO evidence and references
  View Computational GO annotations for UBA4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
250 total interaction(s) for 166 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-Western: 1
  • Biochemical Activity: 5
  • Reconstituted Complex: 1
  • Two-hybrid: 27

Genetic Interactions
  • Negative Genetic: 136
  • Phenotypic Enhancement: 1
  • Positive Genetic: 15
  • Synthetic Growth Defect: 42
  • Synthetic Lethality: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 440
Molecular Weight (Da) 49,361
Isoelectric Point (pI) 6.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:333072 to 334394 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1323 333072..334394 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001153
References cited on this page View Complete Literature Guide for UBA4
1) Furukawa K, et al.  (2000) A protein conjugation system in yeast with homology to biosynthetic enzyme reaction of prokaryotes. J Biol Chem 275(11):7462-5
2) Goehring AS, et al.  (2003) Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p. Eukaryot Cell 2(5):930-6
3) Goehring AS, et al.  (2003) Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast. Mol Biol Cell 14(11):4329-41
4) Noma A, et al.  (2009) Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res 37(4):1335-52
5) Leidel S, et al.  (2009) Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature 458(7235):228-32