Standard Name	UBA3
Systematic Name	YPR066W
Feature Type	ORF, Verified
Description	Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern (1, 2)
Name Description	UBiquitin-like protein-Activating enzyme 1

Chromosomal Location	ChrXVI:681213 to 682112 | ORF Map | GBrowse

	View Computational GO annotations for UBA3
Molecular Function
Manually curated	NEDD8 activating enzyme activity (IDA, ISS)
Biological Process
Manually curated	protein neddylation (IDA, IMP, ISS)
Cellular Component
High-throughput	cytoplasm (IDA)

Classical genetics
null	Ptr2p-GFP accumulation: decreased
Large-scale survey
null	anaerobic growth: decreased competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased nutrient utilization: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to sirolimus: increased resistance to tunicamycin: increased resistance to camptothecin: decreased resistance to mycophenolic acid: decreased resistance to cycloheximide: increased respiratory growth: decreased rate respiratory growth: absent sporulation: absent sporulation efficiency: decreased stress resistance: increased toxin resistance: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	128 total interaction(s) for 97 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Reconstituted Complex: 1 Two-hybrid: 4
Genetic Interactions	Negative Genetic: 77 Positive Genetic: 35 Synthetic Growth Defect: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:681213 to 682112 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..900 681213..682112 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006270