Standard Name	TYW1 1
Systematic Name	YPL207W
Feature Type	ORF, Verified
Description	Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron (2, 3)
Name Description	Trna-YW synthesizing protein 1

Chromosomal Location	ChrXVI:159909 to 162341 | ORF Map | GBrowse

	View Computational GO annotations for TYW1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	wybutosine biosynthetic process (IMP)
Cellular Component
High-throughput	endoplasmic reticulum (IDA)

Classical genetics
overexpression	metal resistance: decreased metal resistance: increased vegetative growth: decreased
Large-scale survey
null	competitive fitness: increased resistance to cycloheximide: decreased resistance to neomycin: decreased sporulation: normal vacuolar morphology: abnormal viable
overexpression	inviable resistance to sirolimus: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	56 total interaction(s) for 42 unique genes/features.
Physical Interactions	Affinity Capture-MS: 14 Affinity Capture-RNA: 2 Biochemical Activity: 1 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 32 Positive Genetic: 2 Synthetic Growth Defect: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:159909 to 162341 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2433 159909..162341 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006128