TSC13/YDL015C Summary Help

Standard Name TSC13 1
Systematic Name YDL015C
Feature Type ORF, Verified
Description Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress (2, 3)
Name Description Temperature-sensitive Suppressors of Csg2 mutants 1
Chromosomal Location
ChrIV:426934 to 426002 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TSC13 GO evidence and references
  View Computational GO annotations for TSC13
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Pathways
Classical genetics
conditional
null
reduction of function
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
160 total interaction(s) for 138 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-Western: 4
  • Co-localization: 1
  • PCA: 39
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 68
  • Positive Genetic: 26
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 310
Molecular Weight (Da) 36,768
Isoelectric Point (pI) 10.38
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:426934 to 426002 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..933 426934..426002 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002173
References cited on this page View Complete Literature Guide for TSC13
1) Beeler T, et al.  (1998) The Saccharomyces cerevisiae TSC10/YBR265w gene encoding 3-ketosphinganine reductase is identified in a screen for temperature-sensitive suppressors of the Ca2+-sensitive csg2Delta mutant. J Biol Chem 273(46):30688-94
2) Kohlwein SD, et al.  (2001) Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae. Mol Cell Biol 21(1):109-25
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76