TSC11/YER093C Summary Help

Standard Name TSC11
Systematic Name YER093C
Alias AVO3
Feature Type ORF, Verified
Description Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description Temperature-sensitive Suppressors of Csg2 mutants 1
Chromosomal Location
ChrV:347612 to 343320 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TSC11 GO evidence and references
  View Computational GO annotations for TSC11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All TSC11 Phenotype evidence and references
Classical genetics
conditional
Large-scale survey
null
overexpression
Resources
interactions All TSC11 Interaction evidence and references
232 total interaction(s) for 179 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 13
  • Co-fractionation: 1
  • Two-hybrid: 98
Genetic Interactions
  • Dosage Rescue: 11
  • Negative Genetic: 63
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 7
  • Positive Genetic: 3
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 5
  • Synthetic Rescue: 9
Resources
Expression Summary
histogram
Resources
Protein Information All TSC11 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:347612 to 343320 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..4293 347612..343320 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000895
SUMMARY PARAGRAPH for TSC11

TSC11 encodes an essential protein that is a subunit of the TOR complex 2 (TORC2) (2). TORC2 is involved in regulating actin cytoskeleton polarization during cell cycle progression, cell wall integrity, and nutrient receptor endocytosis (6, 7). In addition to Tsc11p, TORC2 contains Avo1p, Avo2p, Lst8p, Bit61p, Slm1p, Slm2p, and Tor2p (2, 8). Tsc11p binds to the N-terminal HEAT (Huntingtin, Elongation factor 3, regulatory subunit A of PP2A, TOR1) repeat region of Tor2p and this interaction is necessary to maintain the structural integrity of TORC2 (9). Tor2p is also able to phosphorylate sites on Tsc11p, but the autophosphorylation function of TORC2 is not essential to its integrity (9). A RasGEFN domain, found in exchange factors and activating proteins for Ras-like small GTPases, is present in Tsc11p (2).

TSC11, originally identified as a suppressor of a mutation in CSG2 (1), is conserved from yeast to man and is the homolog of the mammalian protein rictor/mAVO3 (10, 11). Mutants in pianissimo, the Dictyostelium discoideum homolog of Tsc11p, are defective in receptor/G protein-mediated activation of adenylate cyclase (12). TORC2 is also structurally conserved in higher eukaryotes and, like yeast TORC2, functions to regulate the actin cytoskeleton (2, 11).

Last updated: 2006-10-12

References cited on this page View Complete Literature Guide for TSC11
1) Beeler T, et al.  (1998) The Saccharomyces cerevisiae TSC10/YBR265w gene encoding 3-ketosphinganine reductase is identified in a screen for temperature-sensitive suppressors of the Ca2+-sensitive csg2Delta mutant. J Biol Chem 273(46):30688-94
2) Loewith R, et al.  (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10(3):457-68
3) Jacinto E and Hall MN  (2003) Tor signalling in bugs, brain and brawn. Nat Rev Mol Cell Biol 4(2):117-26
4) Wedaman KP, et al.  (2003) Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae. Mol Biol Cell 14(3):1204-20
5) Ho HL, et al.  (2005) Saccharomyces cerevisiaeTSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex. Curr Genet 47(5):273-88
6) Schmidt A, et al.  (1996) TOR2 is required for organization of the actin cytoskeleton in yeast. Proc Natl Acad Sci U S A 93(24):13780-5
7) deHart AK, et al.  (2003) Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway. Mol Biol Cell 14(11):4676-84
8) Reinke A, et al.  (2004) TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem 279(15):14752-62
9) Wullschleger S, et al.  (2005) Molecular organization of target of rapamycin complex 2. J Biol Chem 280(35):30697-704
10) Sarbassov DD, et al.  (2004) Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Curr Biol 14(14):1296-302
11) Jacinto E, et al.  (2004) Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat Cell Biol 6(11):1122-8
12) Chen MY, et al.  (1997) A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium. Genes Dev 11(23):3218-31