TSA1/YML028W Summary Help

Standard Name TSA1 1
Systematic Name YML028W
Alias ZRG14 2 , TPX1
Feature Type ORF, Verified
Description Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form high-molecular weight chaperone complex under oxidative stress; deletion causes mutator phenotype; protein abundance increases and forms cytoplasmic foci during DNA replication stress; chaperone activity is essential for growth under zinc deficiency; required for telomere length maintenance; TSA1 has a paralog, TSA2, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
Name Description Thiol-Specific Antioxidant 1
Gene Product Alias cTPxI
Chromosomal Location
ChrXIII:220138 to 220728 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TSA1 GO evidence and references
  View Computational GO annotations for TSA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 13 genes
Resources
Classical genetics
gain of function
null
reduction of function
Large-scale survey
null
overexpression
Resources
412 total interaction(s) for 234 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 34
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 3
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 3
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 3
  • Negative Genetic: 135
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 5
  • Positive Genetic: 6
  • Synthetic Growth Defect: 115
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 64
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 196
Molecular Weight (Da) 21,590
Isoelectric Point (pI) 4.87
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:220138 to 220728 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..591 220138..220728 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004490
References cited on this page View Complete Literature Guide for TSA1
1) Chae HZ, et al.  (1993) Cloning, sequencing, and mutation of thiol-specific antioxidant gene of Saccharomyces cerevisiae. J Biol Chem 268(22):16815-21
2) Yuan DS  (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58
3) Chae HZ, et al.  (1994) Thioredoxin-dependent peroxide reductase from yeast. J Biol Chem 269(44):27670-8
4) Wong CM, et al.  (2002) Cooperation of yeast peroxiredoxins Tsa1p and Tsa2p in the cellular defense against oxidative and nitrosative stress. J Biol Chem 277(7):5385-94
5) Huang ME, et al.  (2003) A genomewide screen in Saccharomyces cerevisiae for genes that suppress the accumulation of mutations. Proc Natl Acad Sci U S A 100(20):11529-34
6) Wong CM, et al.  (2004) Peroxiredoxin-null yeast cells are hypersensitive to oxidative stress and are genomically unstable. J Biol Chem 279(22):23207-13
7) Okazaki S, et al.  (2005) Peroxiredoxin-mediated redox regulation of the nuclear localization of Yap1, a transcription factor in budding yeast. Antioxid Redox Signal 7(3-4):327-34
8) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9) Trotter EW, et al.  (2008) The yeast Tsa1 peroxiredoxin is a ribosome-associated antioxidant. Biochem J 412(1):73-80
10) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
11) Lu J, et al.  (2013) Deletion of the major peroxiredoxin Tsa1 alters telomere length homeostasis. Aging Cell 12(4):635-44
12) Macdiarmid CW, et al.  (2013) Peroxiredoxin Chaperone Activity Is Critical for Protein Homeostasis in Zinc-deficient Yeast. J Biol Chem 288(43):31313-27