TRS85/YDR108W Summary Help

Standard Name TRS85 1
Systematic Name YDR108W
Alias MUM1 2 , GSG1 3
Feature Type ORF, Verified
Description Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role (2, 3, 4, 5, 6)
Name Description TRapp Subunit 1
Chromosomal Location
ChrIV:671269 to 673365 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TRS85 GO evidence and references
  View Computational GO annotations for TRS85
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Resources
558 total interaction(s) for 273 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 34
  • Affinity Capture-Western: 16
  • Co-fractionation: 1
  • Co-localization: 1
  • PCA: 3
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 342
  • Phenotypic Enhancement: 68
  • Phenotypic Suppression: 14
  • Positive Genetic: 46
  • Synthetic Growth Defect: 19
  • Synthetic Lethality: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 698
Molecular Weight (Da) 80,494
Isoelectric Point (pI) 6.38
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:671269 to 673365 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2097 671269..673365 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002515
References cited on this page View Complete Literature Guide for TRS85
1) Sacher M, et al.  (2000) Identification and characterization of five new subunits of TRAPP. Eur J Cell Biol 79(2):71-80
2) Engebrecht J, et al.  (1998) Yeast meiotic mutants proficient for the induction of ectopic recombination. Genetics 148(2):581-98
3) Kaytor MD and Livingston DM  (1995) GSG1, a yeast gene required for sporulation. Yeast 11(12):1147-55
4) Meiling-Wesse K, et al.  (2005) Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway. J Biol Chem 280(39):33669-78
5) Nazarko TY, et al.  (2005) Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae. Autophagy 1(1):37-45
6) Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6