Standard Name	TRS65 1
Systematic Name	YGR166W
Alias	KRE11 2
Feature Type	ORF, Verified
Description	Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response (1, 2, 3, 4)
Name Description	TRapp Subunit 1

Chromosomal Location	ChrVII:830515 to 832197 | ORF Map | GBrowse
	Genetic position: 154.34 cM

	View Computational GO annotations for TRS65
Molecular Function
Manually curated	contributes_to Rab guanyl-nucleotide exchange factor activity (IGI)
Biological Process
Manually curated	intra-Golgi vesicle-mediated transport (IGI, IPI) protein complex assembly (IGI)
Cellular Component
Manually curated	trans-Golgi network (IDA) TRAPP complex (IDA)

Classical genetics
null	(1->6)-beta-D-glucan accumulation: decreased killer toxin resistance: increased Trs130p-HA accumulation: decreased Ypt31p distribution: abnormal Tca17p-13Myc distribution: abnormal Snc1p-GFP distribution: abnormal GFP-Ypt31p distribution: abnormal resistance to enzymatic treatment: decreased
unspecified	(1->6)-beta-D-glucan accumulation: decreased killer toxin resistance: increased
Large-scale survey
null	auxotrophy competitive fitness: increased haploproficient resistance to cycloheximide: decreased resistance to PM02734: decreased resistance to ethanol: decreased resistance to (S)-lactic acid: decreased resistance to hydrogen chloride: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to chloroquine: decreased resistance to methyl methanesulfonate: decreased resistance to elesclomol-Cu: decreased resistance to hydroxyurea: increased resistance to calcium dichloride: decreased resistance to glycolaldehyde: decreased viable
overexpression	vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	202 total interaction(s) for 112 unique genes/features.
Physical Interactions	Affinity Capture-MS: 17 Affinity Capture-RNA: 1 Affinity Capture-Western: 14 Co-fractionation: 1 Protein-RNA: 1 Two-hybrid: 8
Genetic Interactions	Dosage Rescue: 6 Negative Genetic: 69 Phenotypic Enhancement: 16 Phenotypic Suppression: 6 Positive Genetic: 6 Synthetic Growth Defect: 31 Synthetic Lethality: 26
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:830515 to 832197 | ORF Map | GBrowse
	Genetic position: 154.34 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1683 830515..832197 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003398