TRS20/YBR254C Summary Help

Standard Name TRS20 1
Systematic Name YBR254C
Feature Type ORF, Verified
Description Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); mutation of conserved residues leads to defects in endocytic recycling and a block in sporulation/meiosis; mutations in the human homolog cause the spondyloepiphyseal dysplasia (1, 2, 3, 4, 5)
Name Description TRapp Subunit 1
Chromosomal Location
ChrII:724263 to 723736 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TRS20 GO evidence and references
  View Computational GO annotations for TRS20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
repressible
Resources
277 total interaction(s) for 127 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 39
  • Affinity Capture-Western: 19
  • Co-fractionation: 2
  • Co-purification: 1
  • Two-hybrid: 13

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 172
  • Positive Genetic: 20
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 175
Molecular Weight (Da) 19,700
Isoelectric Point (pI) 7.06
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:724263 to 723736 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..528 724263..723736 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000458
References cited on this page View Complete Literature Guide for TRS20
1) Sacher M, et al.  (2000) Identification and characterization of five new subunits of TRAPP. Eur J Cell Biol 79(2):71-80
2) Sacher M, et al.  (2001) TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport. Mol Cell 7(2):433-42
3) Tiller GE, et al.  (2001) A recurrent RNA-splicing mutation in the SEDL gene causes X-linked spondyloepiphyseal dysplasia tarda. Am J Hum Genet 68(6):1398-407
4) Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6
5) Mahfouz H, et al.  (2012) Mutational Analysis of the Yeast TRAPP Subunit Trs20p Identifies Roles in Endocytic Recycling and Sporulation. PLoS One 7(9):e41408