Standard Name	TRM9
Systematic Name	YML014W
Alias	KTI1 1
Feature Type	ORF, Verified
Description	tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses (2, 3, 4, 5)
Name Description	TRna Methyltransferase 2

Chromosomal Location	ChrXIII:243225 to 244064 | ORF Map | GBrowse

	View Computational GO annotations for TRM9
Molecular Function
Manually curated	tRNA (uracil) methyltransferase activity (IDA)
Biological Process
Manually curated	chronological cell aging (IMP) response to stress (IMP) tRNA methylation (IDA) tRNA wobble uridine modification (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA)
High-throughput	cytoplasm (IDA)

Classical genetics
null	tRNAArg(UCU) modification: decreased tRNAGlu(UUC) modification: decreased tRNAPro(UGG) modification: decreased colony appearance: abnormal Rnr3p accumulation: decreased Yef3p accumulation: decreased Rnr1p accumulation: decreased resistance to hydroxyurea: decreased resistance to paromomycin: decreased resistance to geneticin: decreased resistance to methyl methanesulfonate: decreased toxin resistance: increased
reduction of function	toxin resistance: increased
unspecified	killer toxin resistance: increased
Large-scale survey
null	tRNA modification: abnormal auxotrophy bud morphology: abnormal cell size: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased fermentative growth: decreased rate filamentous growth: decreased haploinsufficient heat sensitivity: increased invasive growth: absent Msb2p-HA secretion: decreased resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to wortmannin: normal resistance to benzo[a]pyrene: decreased resistance to sirolimus: decreased resistance to hygromycin B: decreased resistance to arsenite(3-): decreased resistance to ethanol: decreased stress resistance: increased toxin resistance: increased vacuolar morphology: abnormal vegetative growth: decreased rate vegetative growth: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	75 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 1 Affinity Capture-Western: 4 Reconstituted Complex: 1 Two-hybrid: 2
Genetic Interactions	Negative Genetic: 35 Phenotypic Suppression: 2 Positive Genetic: 14 Synthetic Growth Defect: 9 Synthetic Lethality: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIII:243225 to 244064 | ORF Map | GBrowse
Last Update	Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..840 243225..244064 1996-07-31 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004476