TRM8/YDL201W Summary Help

Standard Name TRM8 1
Systematic Name YDL201W
Feature Type ORF, Verified
Description Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p (1, 2, 3, 4)
Name Description Transfer RNA Methyltransferase 1
Chromosomal Location
ChrIV:99561 to 100421 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TRM8 GO evidence and references
  View Computational GO annotations for TRM8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 75 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
76 total interaction(s) for 48 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 3
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Reconstituted Complex: 2

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 16
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 7
  • Positive Genetic: 8
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 4
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 286
Molecular Weight (Da) 33,391
Isoelectric Point (pI) 9.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:99561 to 100421 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..861 99561..100421 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002360
References cited on this page View Complete Literature Guide for TRM8
1) Alexandrov A, et al.  (2002) Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA 8(10):1253-66
2) Matsumoto K, et al.  (2007) RNA recognition mechanism of eukaryote tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex). FEBS Lett 581(8):1599-604
3) Matsumoto K, et al.  (2008) Production of yeast tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system. J Biotechnol 133(4):453-60
4) Leulliot N, et al.  (2008) Structure of the Yeast tRNA m7G Methylation Complex. Structure 16(1):52-61