TRM2/YKR056W Summary Help

Standard Name TRM2
Systematic Name YKR056W
Alias NUC2 , NUD1 , RNC1
Feature Type ORF, Verified
Description tRNA methyltransferase; 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair (1, 2, 3)
Name Description Transfer RNA Methyltransferase
Chromosomal Location
ChrXI:549448 to 551367 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TRM2 GO evidence and references
  View Computational GO annotations for TRM2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
44 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1

Genetic Interactions
  • Negative Genetic: 26
  • Phenotypic Enhancement: 4
  • Positive Genetic: 5
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 639
Molecular Weight (Da) 72,852
Isoelectric Point (pI) 9.27
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:549448 to 551367 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2004-02-13
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1920 549448..551367 2011-02-03 2004-02-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001764
References cited on this page View Complete Literature Guide for TRM2
1) Nordlund ME, et al.  (2000) Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae. RNA 6(6):844-60
2) Johansson MJ and Bystrom AS  (2002) Dual function of the tRNA(m(5)U54)methyltransferase in tRNA maturation. RNA 8(3):324-35
3) Choudhury SA, et al.  (2007) Functional and genetic analysis of the Saccharomyces cerevisiae RNC1/TRM2: evidences for its involvement in DNA double-strand break repair. Mol Cell Biochem 300(1-2):215-26