TRM112/YNR046W Summary Help

Standard Name TRM112 1
Systematic Name YNR046W
Feature Type ORF, Verified
Description Protein involved in methylation of tRNA, rRNA, and translation factors; also involved in ribosome biogenesis; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description TRna Methyltransferase 1
Chromosomal Location
ChrXIV:707788 to 708195 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TRM112 GO evidence and references
  View Computational GO annotations for TRM112
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
repressible
Large-scale survey
null
reduction of function
repressible
Resources
97 total interaction(s) for 72 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 60
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 10
  • Co-purification: 1
  • PCA: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 4

Genetic Interactions
  • Negative Genetic: 2
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 135
Molecular Weight (Da) 15,066
Isoelectric Point (pI) 4.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:707788 to 708195 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..408 707788..708195 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005329
References cited on this page View Complete Literature Guide for TRM112
1) Purushothaman SK, et al.  (2005) Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA. Mol Cell Biol 25(11):4359-70
2) Heurgue-Hamard V, et al.  (2006) The Zinc Finger Protein Ynr046w Is Plurifunctional and a Component of the eRF1 Methyltransferase in Yeast. J Biol Chem 281(47):36140-8
3) Studte P, et al.  (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77
4) Figaro S, et al.  (2012) Trm112 is required for Bud23-mediated methylation of the 18S rRNA at position G1575. Mol Cell Biol 32(12):2254-67
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Sardana R and Johnson AW  (2012) The methyltransferase adaptor protein Trm112 is involved in biogenesis of both ribosomal subunits. Mol Biol Cell 23(21):4313-22