TPT1/YOL102C Summary Help

Standard Name TPT1
Systematic Name YOL102C
Feature Type ORF, Verified
Description tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide (1 and see Summary Paragraph)
Gene Product Alias tRNA 2'-phosphotransferase 2
Chromosomal Location
ChrXV:126689 to 125997 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TPT1 GO evidence and references
  View Computational GO annotations for TPT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All TPT1 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
reduction of function
Resources
interactions All TPT1 Interaction evidence and references
11 total interaction(s) for 11 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Two-hybrid: 1
Genetic Interactions
  • Synthetic Lethality: 3
Resources
Expression Summary
histogram
Resources
Protein Information All TPT1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:126689 to 125997 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..693 126689..125997 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005462
SUMMARY PARAGRAPH for TPT1

TPT1 is an essential gene that encodes tRNA 2'-phosphotransferase, which catalyzes the final step of tRNA splicing (2). In tRNA processing, an intron is removed from the tRNA transcript by the multisubunit tRNA splicing endonuclease (comprising Sen2p, Sen15p, Sen34p, and Sen54p); the resulting half-molecules are joined by the tRNA ligase Trl1p (reviewed in reference 3). Tpt1p catalyzes the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1"-2" cyclic phosphate (2). In a conditional lethal tpt1 mutant, 2'-phosphorylated tRNAs accumulate, and the tRNAs show altered posttranscriptional modification (4). Enzymes similar to Tpt1p are found in other eukaryotes; a predicted protein from S. pombe shows considerable sequence similarity to Tpt1p (2, 5). E. coli also contains a Tpt1p homolog, although it does not have the class of tRNA splicing found in eukaryotes (5).

Last updated: 2000-04-18

References cited on this page View Complete Literature Guide for TPT1
1) Sawaya R, et al.  (2005) Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1. RNA 11(1):107-13
2) Culver GM, et al.  (1997) A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae. J Biol Chem 272(20):13203-10
3) Abelson J, et al.  (1998) tRNA splicing. J Biol Chem 273(21):12685-8
4) Spinelli SL, et al.  (1997) A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction. RNA 3(12):1388-400
5) Spinelli SL, et al.  (1999) Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast. J Biol Chem 274(5):2637-44