TPM1/YNL079C Summary Help

Standard Name TPM1
Systematic Name YNL079C
Feature Type ORF, Verified
Description Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description TroPoMyosin
Gene Product Alias tropomyosin
Chromosomal Location
ChrXIV:479165 to 478566 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TPM1 GO evidence and references
  View Computational GO annotations for TPM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
363 total interaction(s) for 254 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 236
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 10
  • Positive Genetic: 26
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 27
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 199
Molecular Weight (Da) 23,541
Isoelectric Point (pI) 4.4
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:479165 to 478566 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..600 479165..478566 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005023
References cited on this page View Complete Literature Guide for TPM1
1) Liu HP and Bretscher A  (1989) Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton. Cell 57(2):233-42
2) Pruyne D and Bretscher A  (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
3) Singer JM and Shaw JM  (2003) Mdm20 protein functions with Nat3 protein to acetylate Tpm1 protein and regulate tropomyosin-actin interactions in budding yeast. Proc Natl Acad Sci U S A 100(13):7644-9
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61