TOM1/YDR457W Summary Help

Standard Name TOM1 1
Systematic Name YDR457W
Feature Type ORF, Verified
Description E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase (2, 3, 4, 5, 6, 7, 8)
Name Description Temperature dependent Organization in Mitotic nucleus or Trigger Of Mitosis 1, 2
Chromosomal Location
ChrIV:1369790 to 1379596 | ORF Map | GBrowse
Gene Ontology Annotations All TOM1 GO evidence and references
  View Computational GO annotations for TOM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 3 genes
Classical genetics
Large-scale survey
180 total interaction(s) for 158 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 26
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • Co-purification: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 11
  • Negative Genetic: 93
  • Phenotypic Enhancement: 3
  • Positive Genetic: 13
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 5
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 3,268
Molecular Weight (Da) 374,180
Isoelectric Point (pI) 4.92
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1369790 to 1379596 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..9807 1369790..1379596 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002865
References cited on this page View Complete Literature Guide for TOM1
1) Utsugi T, et al.  (1995) A high dose of the STM1 gene suppresses the temperature sensitivity of the tom1 and htr1 mutants in Saccharomyces cerevisiae. Biochim Biophys Acta 1263(3):285-8
2) Utsugi T, et al.  (1999) Yeast tom1 mutant exhibits pleiotropic defects in nuclear division, maintenance of nuclear structure and nucleocytoplasmic transport at high temperatures. Gene 234(2):285-95
3) Davey M, et al.  (2000) The yeast peptidyl proline isomerases FPR3 and FPR4, in high copy numbers, suppress defects resulting from the absence of the E3 ubiquitin ligase TOM1. Mol Gen Genet 263(3):520-6
4) Saleh A, et al.  (1998) TOM1p, a yeast hect-domain protein which mediates transcriptional regulation through the ADA/SAGA coactivator complexes. J Mol Biol 282(5):933-46
5) Duncan K, et al.  (2000) A putative ubiquitin ligase required for efficient mRNA export differentially affects hnRNP transport. Curr Biol 10(12):687-96
6) Singh RK, et al.  (2009) Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis. Nat Cell Biol 11(8):925-33
7) Kim DH and Koepp DM  (2012) Hect E3 ubiquitin ligase Tom1 controls Dia2 degradation during the cell cycle. Mol Biol Cell 23(21):4203-11
8) Kim DH, et al.  (2012) The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase. J Biol Chem 287(53):44212-20