Standard Name	TOF2 1
Systematic Name	YKR010C
Feature Type	ORF, Verified
Description	Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description	TOpoisomerase I-interacting Factor 1

Chromosomal Location	ChrXI:461239 to 458924 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for TOF2
Molecular Function
Manually curated	phosphatase activator activity (IDA)
Biological Process
Manually curated	chromatin silencing at rDNA (IMP) nucleolus organization (IGI, IMP) protein localization to nucleolar rDNA repeats (IMP) rDNA condensation (IMP)
Cellular Component
Manually curated	nucleolus (IDA)
High-throughput	mitochondrion (IDA)

Classical genetics
null	resistance to trichothecene: increased resistance to (-)-anisomycin: increased resistance to chloramphenicol: increased viable
Large-scale survey
null	competitive fitness: decreased filamentous growth: decreased invasive growth: absent Rad52-YFP distribution: increased resistance to bleomycin: decreased resistance to trichothecene: increased resistance to fluconazole: decreased resistance to fenpropimorph: decreased resistance to caffeine: decreased resistance to camptothecin: decreased resistance to methyl methanesulfonate: decreased resistance to cycloheximide: increased resistance to cycloheximide: decreased resistance to amitrole: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	144 total interaction(s) for 92 unique genes/features.
Physical Interactions	Affinity Capture-MS: 30 Affinity Capture-RNA: 3 Affinity Capture-Western: 4 Reconstituted Complex: 1 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 74 Positive Genetic: 10 Synthetic Growth Defect: 7 Synthetic Lethality: 6 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:461239 to 458924 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2316 461239..458924 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001718