TOA2/YKL058W Summary Help

Standard Name TOA2 1
Systematic Name YKL058W
Feature Type ORF, Verified
Description TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa1p, for ribosomal protein gene transcription in vivo; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress (2, 3, 4)
Chromosomal Location
ChrXI:330166 to 330534 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TOA2 GO evidence and references
  View Computational GO annotations for TOA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 6 genes
Regulators 5 genes
Resources
Classical genetics
conditional
null
reduction of function
repressible
Large-scale survey
null
Resources
66 total interaction(s) for 33 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Co-crystal Structure: 5
  • Co-purification: 2
  • PCA: 1
  • Reconstituted Complex: 15
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 8
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 122
Molecular Weight (Da) 13,458
Isoelectric Point (pI) 4.75
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:330166 to 330534 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..369 330166..330534 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001541
References cited on this page View Complete Literature Guide for TOA2
1) Ranish JA, et al.  (1992) Isolation of two genes that encode subunits of the yeast transcription factor IIA. Science 255(5048):1127-9
2) Hampsey M  (1998) Molecular genetics of the RNA polymerase II general transcriptional machinery. Microbiol Mol Biol Rev 62(2):465-503
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Layer JH and Weil PA  (2013) Direct TFIIA-TFIID Protein Contacts Drive Budding Yeast Ribosomal Protein Gene Transcription. J Biol Chem 288(32):23273-94