CDC55/YGL190C Summary Help

Standard Name CDC55 1
Systematic Name YGL190C
Alias TMR4 2
Feature Type ORF, Verified
Description Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; homolog of mammalian B55 (3, 4, 5, 6, 7, 8, 9)
Name Description Cell Division Cycle
Chromosomal Location
ChrVII:147389 to 145809 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -125 cM
Gene Ontology Annotations All CDC55 GO evidence and references
  View Computational GO annotations for CDC55
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
conditional
null
repressible
unspecified
Large-scale survey
null
overexpression
Resources
299 total interaction(s) for 194 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 77
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 19
  • Biochemical Activity: 5
  • Protein-RNA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 8
  • Dosage Rescue: 5
  • Negative Genetic: 42
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 22
  • Positive Genetic: 44
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 14
  • Synthetic Rescue: 19

Resources
Expression Summary
histogram
Resources
Length (a.a.) 526
Molecular Weight (Da) 59,662
Isoelectric Point (pI) 6.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:147389 to 145809 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -125 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1581 147389..145809 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003158
References cited on this page View Complete Literature Guide for CDC55
1) Healy AM, et al.  (1991) CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase. Mol Cell Biol 11(11):5767-80
2) Rossio V, et al.  (2013) Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint. J Cell Sci 126(Pt 19):4396-4405
3) Jiang Y and Broach JR  (1999) Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast. EMBO J 18(10):2782-92
4) Wang Y and Burke DJ  (1997) Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol Cell Biol 17(2):620-6
5) Gentry MS and Hallberg RL  (2002) Localization of Saccharomyces cerevisiae protein phosphatase 2A subunits throughout mitotic cell cycle. Mol Biol Cell 13(10):3477-92
6) Queralt E, et al.  (2006) Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast. Cell 125(4):719-32
7) Rossio V and Yoshida S  (2011) Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast. J Cell Biol 193(3):445-54
8) Bizzari F and Marston AL  (2011) Cdc55 coordinates spindle assembly and chromosome disjunction during meiosis. J Cell Biol 193(7):1213-28
9) Mui MZ, et al.  (2013) Identification of the adenovirus E4orf4 protein binding site on the B55alpha and Cdc55 regulatory subunits of PP2A: Implications for PP2A function, tumor cell killing and viral replication. PLoS Pathog 9(11):e1003742