TLG2/YOL018C Summary Help

Standard Name TLG2 1
Systematic Name YOL018C
Feature Type ORF, Verified
Description Syntaxin-like t-SNARE; forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) (2, 3, 4, 5)
Name Description T-snare affecting a Late Golgi compartment 1
Chromosomal Location
ChrXV:292074 to 290881 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TLG2 GO evidence and references
  View Computational GO annotations for TLG2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
860 total interaction(s) for 395 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 23
  • Biochemical Activity: 2
  • Co-purification: 35
  • PCA: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 577
  • Phenotypic Enhancement: 85
  • Phenotypic Suppression: 12
  • Positive Genetic: 61
  • Synthetic Growth Defect: 33
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 397
Molecular Weight (Da) 45,875
Isoelectric Point (pI) 4.98
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:292074 to 290881 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1194 292074..290881 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005378
References cited on this page View Complete Literature Guide for TLG2
1) Holthuis JC, et al.  (1998) Two syntaxin homologues in the TGN/endosomal system of yeast. EMBO J 17(1):113-26
2) Coe JG, et al.  (1999) A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae. Mol Biol Cell 10(7):2407-23
3) Bryant NJ and James DE  (2001) Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation. EMBO J 20(13):3380-8
4) Dulubova I, et al.  (2002) How Tlg2p/syntaxin 16 'snares' Vps45. EMBO J 21(14):3620-31
5) Struthers MS, et al.  (2009) Functional homology of mammalian syntaxin 16 and yeast Tlg2p reveals a conserved regulatory mechanism. J Cell Sci 122(Pt 13):2292-9