TIM11/YDR322C-A Summary

Standard Name	TIM11
Systematic Name	YDR322C-A
Alias	ATP21
Feature Type	ORF, Verified
Description	Subunit e of mitochondrial F1F0-ATPase; ATPase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase, which in turn determines the shape of inner membrane cristae (1, 2, 3, 4 and see Summary Paragraph)
Name Description	Translocase of the Inner Mitochondrial membrane

Chromosomal Location	ChrIV:1112293 to 1112003 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All TIM11 GO evidence and references

	View Computational GO annotations for TIM11
Molecular Function
Manually curated	contributes_to proton-transporting ATPase activity, rotational mechanism (IDA) structural molecule activity (IMP, IPI)
Biological Process
Manually curated	ATP synthesis coupled proton transport (IDA, IMP, IPI, ISS) cristae formation (IMP) protein complex oligomerization (IMP)
Cellular Component
Manually curated	mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) (IMP, IPI, ISS)
High-throughput	integral to membrane (ISM) mitochondrion (IDA)

Mutant phenotypes All TIM11 Phenotype evidence and references

Classical genetics
null	mitochondrial morphology: abnormal respiratory growth: decreased respiratory growth: decreased rate
reduction of function	mitochondrial morphology: abnormal
repressible	mitochondrial morphology: abnormal
Large-scale survey
null	resistance to quinine: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All TIM11 Interaction evidence and references

	57 total interaction(s) for 50 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 1 Affinity Capture-Western: 7 Co-purification: 2
Genetic Interactions	Negative Genetic: 33 Phenotypic Enhancement: 1 Positive Genetic: 7 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All TIM11 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1112293 to 1112003 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1999-07-17

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..291	1112293..1112003	2011-02-03	1999-07-17

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000007255

SUMMARY PARAGRAPH for TIM11

TIM11 encodes subunit e of mitochondrial ATP synthase (1). The ATP synthase complex utilizes proton motive force to generate ATP from ADP and P_i (5). The structure of this enzyme complex is highly conserved among diverse organisms and consists of two major components, soluble F₁ and membrane-bound F₀, each of which contains many subunits (6). Subunit e, like subunit g (Atp20p), is not essential for the basic function or assembly of the F₁/F₀ ATP synthase complex, but is required for complex dimerization and maximal enzyme stability. Deletion of TIM11 reduces the mitochondrial concentration of ATP synthase, but does not alter enzyme activity (2).

General ATP synthase structure and function are reviewed in references 5 and 7. For a review that is specific to yeast, see reference 6.

Last updated: 2001-01-18

References cited on this page View Complete Literature Guide for TIM11

1)	Arnold I, et al. (1997) Yeast mitochondrial F1F0-ATPase: the novel subunit e is identical to Tim11. FEBS Lett 411(2-3):195-200
2)	Arnold I, et al. (1998) Yeast mitochondrial F1F0-ATP synthase exists as a dimer: identification of three dimer-specific subunits. EMBO J 17(24):7170-8
3)	Arselin G, et al. (2003) The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane. Eur J Biochem 270(8):1875-84
4)	Davies KM, et al. (2012) Structure of the yeast F1Fo-ATP synthase dimer and its role in shaping the mitochondrial cristae. Proc Natl Acad Sci U S A 109(34):13602-7
5)	Boyer PD (1997) The ATP synthase--a splendid molecular machine. Annu Rev Biochem 66:717-49
6)	Devenish RJ, et al. (2000) Insights into ATP synthase assembly and function through the molecular genetic manipulation of subunits of the yeast mitochondrial enzyme complex. Biochim Biophys Acta 1458(2-3):428-42
7)	Nakamoto RK, et al. (1999) Rotational coupling in the F0F1 ATP synthase. Annu Rev Biophys Biomol Struct 28:205-34