Standard Name	TIF5
Systematic Name	YPR041W
Alias	SUI5 1
Feature Type	ORF, Verified
Description	Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 (2, 3, 4)
Name Description	Translation Initiation Factor

Chromosomal Location	ChrXVI:648704 to 649921 | ORF Map | GBrowse

	View Computational GO annotations for TIF5
Molecular Function
Manually curated	GDP-dissociation inhibitor activity (IDA, IMP) GTPase activator activity (IPI, ISS) translation initiation factor activity (ISS) translation initiation factor binding (IPI)
Biological Process
Manually curated	mature ribosome assembly (IMP) negative regulation of translational initiation (IGI, IMP) regulation of translational initiation (IDA, IMP, IPI)
Cellular Component
Manually curated	cytosolic small ribosomal subunit (IPI) multi-eIF complex (IDA)

Classical genetics
conditional	heat sensitivity: increased resistance to amitrole: decreased vegetative growth: decreased rate
reduction of function	resistance to 3-aminotriazole: decreased
Large-scale survey
null	inviable
overexpression	vegetative growth: decreased
reduction of function	competitive fitness: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	221 total interaction(s) for 86 unique genes/features.
Physical Interactions	Affinity Capture-MS: 82 Affinity Capture-RNA: 3 Affinity Capture-Western: 44 Co-fractionation: 7 Co-purification: 3 Far Western: 1 PCA: 2 Reconstituted Complex: 29 Two-hybrid: 2
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 2 Dosage Rescue: 4 Negative Genetic: 27 Phenotypic Enhancement: 1 Phenotypic Suppression: 3 Positive Genetic: 2 Synthetic Lethality: 5 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:648704 to 649921 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1218 648704..649921 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006245