TIF35/YDR429C Summary Help

Standard Name TIF35 1
Systematic Name YDR429C
Feature Type ORF, Verified
Description eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation (2, 3)
Name Description Translation Initiation Factor 1
Chromosomal Location
ChrIV:1325301 to 1324477 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TIF35 GO evidence and references
  View Computational GO annotations for TIF35
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Large-scale survey
null
reduction of function
repressible
Resources
165 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 71
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 21
  • Co-fractionation: 7
  • Co-purification: 2
  • Reconstituted Complex: 9
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 2
  • Negative Genetic: 23
  • Positive Genetic: 10
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 274
Molecular Weight (Da) 30,501
Isoelectric Point (pI) 6.78
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1325301 to 1324477 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..825 1325301..1324477 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002837
References cited on this page View Complete Literature Guide for TIF35
1) Hanachi P, et al.  (1999) Characterization of the p33 subunit of eukaryotic translation initiation factor-3 from Saccharomyces cerevisiae. J Biol Chem 274(13):8546-53
2) Phan L, et al.  (1998) Identification of a translation initiation factor 3 (eIF3) core complex, conserved in yeast and mammals, that interacts with eIF5. Mol Cell Biol 18(8):4935-46
3) Cuchalova L, et al.  (2010) The RNA Recognition Motif of Eukaryotic Translation Initiation Factor 3g (eIF3g) Is Required for Resumption of Scanning of Posttermination Ribosomes for Reinitiation on GCN4 and Together with eIF3i Stimulates Linear Scanning. Mol Cell Biol 30(19):4671-86