TIF1/YKR059W Summary Help

Standard Name TIF1 1, 2
Systematic Name YKR059W
Feature Type ORF, Verified
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication (3, 4, 5, 6, 7, 8)
Name Description Translation Initiation Factor 1
Gene Product Alias eIF4A
Chromosomal Location
ChrXI:554987 to 556174 | ORF Map | GBrowse
Genetic position: 22 cM
Gene Ontology Annotations All TIF1 GO evidence and references
  View Computational GO annotations for TIF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
Large-scale survey
167 total interaction(s) for 122 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 50
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 6
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • PCA: 1
  • Protein-RNA: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 9
  • Negative Genetic: 39
  • Positive Genetic: 30
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 6
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 395
Molecular Weight (Da) 44,697
Isoelectric Point (pI) 4.85
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:554987 to 556174 | ORF Map | GBrowse
Genetic position: 22 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1188 554987..556174 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001767
References cited on this page View Complete Literature Guide for TIF1
1) Linder P and Slonimski PP  (1989) An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation. Proc Natl Acad Sci U S A 86(7):2286-90
2) Mueller, P. and Linder, P.  (1989) Personal Communication, Mortimer Map Edition 10
3) Blum S, et al.  (1989) Translation in Saccharomyces cerevisiae: initiation factor 4A-dependent cell-free system. Proc Natl Acad Sci U S A 86(16):6043-6
4) Neff CL and Sachs AB  (1999) Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally. Mol Cell Biol 19(8):5557-64
5) Chang TH, et al.  (1990) Identification of five putative yeast RNA helicase genes. Proc Natl Acad Sci U S A 87(4):1571-5
6) Caruthers JM, et al.  (2000) Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase. Proc Natl Acad Sci U S A 97(24):13080-5
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76