NDC80/YIL144W Summary Help

Standard Name NDC80 1
Systematic Name YIL144W
Alias HEC1 2 , TID3 3
Feature Type ORF, Verified
Description Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation (2, 4, 5, 6, 7, 8, 9)
Name Description Nuclear Division Cycle 10
Chromosomal Location
ChrIX:78074 to 80149 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NDC80 GO evidence and references
  View Computational GO annotations for NDC80
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
conditional
overexpression
reduction of function
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
188 total interaction(s) for 92 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 36
  • Affinity Capture-Western: 34
  • Biochemical Activity: 2
  • Co-fractionation: 3
  • Co-localization: 3
  • Co-purification: 8
  • PCA: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 72

Genetic Interactions
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 7
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 691
Molecular Weight (Da) 80,487
Isoelectric Point (pI) 9.39
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:78074 to 80149 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2076 78074..80149 1994-12-10 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001406
References cited on this page View Complete Literature Guide for NDC80
1) Wigge PA, et al.  (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77
2) Zheng L, et al.  (1999) Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins. Mol Cell Biol 19(8):5417-28
3) Dresser ME, et al.  (1997) DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics 147(2):533-44
4) Wigge PA and Kilmartin JV  (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60
5) Janke C, et al.  (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91
6) McCleland ML, et al.  (2003) The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17(1):101-14
7) Westermann S, et al.  (2003) Architecture of the budding yeast kinetochore reveals a conserved molecular core. J Cell Biol 163(2):215-22
8) He X, et al.  (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206
9) Yong-Gonzales V, et al.  (2012) The Smc5-Smc6 Complex Regulates Recombination at Centromeric Regions and Affects Kinetochore Protein Sumoylation during Normal Growth. PLoS One 7(12):e51540
10) Thomas JH and Botstein D  (1986) A gene required for the separation of chromosomes on the spindle apparatus in yeast. Cell 44(1):65-76