THP2/YHR167W Summary Help

Standard Name THP2 1
Systematic Name YHR167W
Feature Type ORF, Verified
Description Subunit of the THO and TREX complexes; THO connects transcription elongation and mitotic recombination, and TREX is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance (1, 2, 3 and see Summary Paragraph)
Name Description THO2 - HPR1 Phenotype 1
Chromosomal Location
ChrVIII:439342 to 440127 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All THP2 GO evidence and references
  View Computational GO annotations for THP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
427 total interaction(s) for 244 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 61
  • Affinity Capture-Western: 10
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 282
  • Phenotypic Enhancement: 1
  • Positive Genetic: 33
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 11
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 261
Molecular Weight (Da) 30,299
Isoelectric Point (pI) 4.82
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:439342 to 440127 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..786 439342..440127 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001210
SUMMARY PARAGRAPH for THP2

THP2 encodes a subunit of the THO complex, a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation by RNA polymerase II (1). Null mutations of any of the four THO complex genes are viable and exhibit similar phenotypes: a defect in transcription elongation, an increase in mitotic recombination between direct repeats (1), and defects in mRNA export (4, 2). The THO complex, along with Sub2p, Yra1p, and Tex1p, forms the transcription export (TREX) complex, conserved from yeast to metazoans and involved in the coupling of transcription to the export of the resulting mRNA (2).

Last updated: 2003-02-25 Contact SGD

References cited on this page View Complete Literature Guide for THP2
1) Chavez S, et al.  (2000) A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO J 19(21):5824-34
2) Strasser K, et al.  (2002) TREX is a conserved complex coupling transcription with messenger RNA export. Nature 417(6886):304-8
3) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
4) Jimeno S, et al.  (2002) The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability. EMBO J 21(13):3526-35