THO2/YNL139C Summary Help

Standard Name THO2 1
Systematic Name YNL139C
Alias ZRG13 2 , LDB5 , RLR1 3
Feature Type ORF, Verified
Description Subunit of the THO complex; THO is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids (1, 3, 4, 5)
Name Description suppressor of the Transcriptional defect of Hpr1 by Overexpression 1
Chromosomal Location
ChrXIV:365717 to 360924 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All THO2 GO evidence and references
  View Computational GO annotations for THO2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
129 total interaction(s) for 49 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 66
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 17
  • Co-crystal Structure: 4
  • Co-localization: 1
  • Co-purification: 5
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 3
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 13
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,597
Molecular Weight (Da) 183,929
Isoelectric Point (pI) 7.67
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:365717 to 360924 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4794 365717..360924 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005083
References cited on this page View Complete Literature Guide for THO2
1) Piruat JI and Aguilera A  (1998) A novel yeast gene, THO2, is involved in RNA pol II transcription and provides new evidence for transcriptional elongation-associated recombination. EMBO J 17(16):4859-72
2) Yuan DS  (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58
3) West RW Jr, et al.  (2000) RLR1 (THO2), required for expressing lacZ fusions in yeast, is conserved from yeast to humans and is a suppressor of SIN4. Gene 243(1-2):195-205
4) Chavez S, et al.  (2000) A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO J 19(21):5824-34
5) Rondon AG, et al.  (2003) Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation. J Biol Chem 278(40):39037-43