THI20/YOL055C Summary Help

Standard Name THI20 1
Systematic Name YOL055C
Feature Type ORF, Verified
Description Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p (1, 2, 3)
Name Description THIamine metabolism 1
Chromosomal Location
ChrXV:226075 to 224420 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All THI20 GO evidence and references
  View Computational GO annotations for THI20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Pathways
Large-scale survey
null
overexpression
Resources
17 total interaction(s) for 17 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 6
  • Phenotypic Enhancement: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Haploinsufficiency: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 551
Molecular Weight (Da) 61,269
Isoelectric Point (pI) 6.1
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:226075 to 224420 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1656 226075..224420 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005416
References cited on this page View Complete Literature Guide for THI20
1) Llorente B, et al.  (1999) Genetic redundancy and gene fusion in the genome of the Baker's yeast Saccharomyces cerevisiae: functional characterization of a three-member gene family involved in the thiamine biosynthetic pathway. Mol Microbiol 32(6):1140-52
2) Haas AL, et al.  (2005) Thi20, a remarkable enzyme from Saccharomyces cerevisiae with dual thiamin biosynthetic and degradation activities. Bioorg Chem 33(4):338-44
3) French JB, et al.  (2011) Structure of trifunctional THI20 from yeast. Acta Crystallogr D Biol Crystallogr 67(Pt 9):784-91