THG1/YGR024C Summary Help

Standard Name THG1 1
Systematic Name YGR024C
Feature Type ORF, Verified
Description tRNAHis guanylyltransferase; adds a guanosine residue to the 5' end of tRNAH is after transcription and RNase P cleavage; can also catalyze reverse (3'-5') polymerization with certain substrates in a template-dependent reaction; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes (1, 2, 3)
Name Description tRNAHis Guanylyltransferase 1
Chromosomal Location
ChrVII:532596 to 531883 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All THG1 GO evidence and references
  View Computational GO annotations for THG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 3 genes
Resources
Classical genetics
repressible
Large-scale survey
null
Resources
54 total interaction(s) for 51 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 33
  • Positive Genetic: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 237
Molecular Weight (Da) 27,757
Isoelectric Point (pI) 8.21
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:532596 to 531883 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..714 532596..531883 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003256
References cited on this page View Complete Literature Guide for THG1
1) Gu W, et al.  (2003) tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis. Genes Dev 17(23):2889-901
2) Rice TS, et al.  (2005) The highly conserved tRNAHis guanylyltransferase Thg1p interacts with the origin recognition complex and is required for the G2/M phase transition in the yeast Saccharomyces cerevisiae. Eukaryot Cell 4(4):832-5
3) Jackman JE and Phizicky EM  (2006) tRNAHis guanylyltransferase catalyzes a 3'-5' polymerization reaction that is distinct from G-1 addition. Proc Natl Acad Sci U S A 103(23):8640-5