Standard Name	TGS1 1
Systematic Name	YPL157W
Feature Type	ORF, Verified
Description	Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs (1, 2, 3, 4)
Name Description	TrimethylGuanosine Synthase 1

Chromosomal Location	ChrXVI:254814 to 255761 | ORF Map | GBrowse

	View Computational GO annotations for TGS1
Molecular Function
Manually curated	RNA methyltransferase activity (IMP, ISS)
Biological Process
Manually curated	7-methylguanosine cap hypermethylation (IMP) meiosis (IMP) nucleologenesis (IMP) regulation of telomere maintenance via telomerase (IMP) RNA methylation (IMP)
Cellular Component
Manually curated	nucleolus (IDA)

Classical genetics
null	SAE3 mRNA modification: decreased cold sensitivity: increased Gag accumulation: decreased replicative lifespan: decreased silencing: increased sporulation efficiency: decreased telomere length: increased
Large-scale survey
null	auxotrophy cell cycle progression in G1 phase: increased duration competitive fitness: decreased fermentative growth: decreased rate resistance to bleomycin: increased resistance to camptothecin: increased resistance to fluconazole: increased resistance to mycophenolic acid: increased resistance to tunicamycin: increased resistance to sirolimus: decreased resistance to dimethyl sulfoxide: decreased resistance to cycloheximide: increased resistance to benzo[a]pyrene: decreased sporulation: decreased stress resistance: increased transposable element transposition: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	230 total interaction(s) for 195 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Reconstituted Complex: 3 Two-hybrid: 4
Genetic Interactions	Negative Genetic: 123 Phenotypic Enhancement: 2 Phenotypic Suppression: 3 Positive Genetic: 61 Synthetic Growth Defect: 23 Synthetic Lethality: 8 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:254814 to 255761 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..948 254814..255761 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006078