TGS1/YPL157W Summary Help

Standard Name TGS1 1
Systematic Name YPL157W
Feature Type ORF, Verified
Description Trimethyl guanosine synthase, conserved nucleolar methyl transferase; converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs; interacts with Swm2p, which may confer substrate specificity on Tgs1p (1, 2, 3, 4, 5)
Name Description TrimethylGuanosine Synthase 1
Chromosomal Location
ChrXVI:254814 to 255761 | ORF Map | GBrowse
Gene Ontology Annotations All TGS1 GO evidence and references
  View Computational GO annotations for TGS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
235 total interaction(s) for 194 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Co-localization: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 123
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 61
  • Synthetic Growth Defect: 24
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 315
Molecular Weight (Da) 36,527
Isoelectric Point (pI) 10.28
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:254814 to 255761 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..948 254814..255761 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006078
References cited on this page View Complete Literature Guide for TGS1
1) Mouaikel J, et al.  (2002) Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus. Mol Cell 9(4):891-901
2) Colau G, et al.  (2004) The small nucle(ol)ar RNA cap trimethyltransferase is required for ribosome synthesis and intact nucleolar morphology. Mol Cell Biol 24(18):7976-86
3) Franke J, et al.  (2008) Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1. J Cell Sci 121(Pt 21):3553-60
4) Qiu ZR, et al.  (2011) An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs. Nucleic Acids Res 39(13):5633-46
5) Boon KL and Kos M  (2010) Deletion of Swm2p selectively impairs trimethylation of snRNAs by trimethylguanosine synthase (Tgs1p). FEBS Lett 584(15):3299-304