TFG1/YGR186W Summary Help

Standard Name TFG1 1
Systematic Name YGR186W
Alias SSU71 2 , RAP74
Feature Type ORF, Verified
Description TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 (2, 3)
Name Description Transcription Factor G 1
Chromosomal Location
ChrVII:867774 to 869981 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TFG1 GO evidence and references
  View Computational GO annotations for TFG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
null
unspecified
Large-scale survey
null
reduction of function
Resources
183 total interaction(s) for 86 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 97
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 13
  • Biochemical Activity: 3
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Co-purification: 10
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 32
  • Positive Genetic: 3
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 735
Molecular Weight (Da) 82,194
Isoelectric Point (pI) 5.48
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:867774 to 869981 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2208 867774..869981 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003418
References cited on this page View Complete Literature Guide for TFG1
1) Henry NL, et al.  (1994) TFIIF-TAF-RNA polymerase II connection. Genes Dev 8(23):2868-78
2) Sun ZW and Hampsey M  (1995) Identification of the gene (SSU71/TFG1) encoding the largest subunit of transcription factor TFIIF as a suppressor of a TFIIB mutation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 92(8):3127-31
3) Hampsey M  (1998) Molecular genetics of the RNA polymerase II general transcriptional machinery. Microbiol Mol Biol Rev 62(2):465-503