Standard Name	TFG1 1
Systematic Name	YGR186W
Alias	SSU71 2 , RAP74
Feature Type	ORF, Verified
Description	TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 (2, 3)
Name Description	Transcription Factor G 1

Chromosomal Location	ChrVII:867774 to 869981 | ORF Map | GBrowse

	View Computational GO annotations for TFG1
Molecular Function
Manually curated	core RNA polymerase II binding transcription factor activity (IDA) contributes_to core RNA polymerase II recruiting transcription factor activity (IC) protein phosphatase activator activity (IDA)
Biological Process
Manually curated	dephosphorylation of RNA polymerase II C-terminal domain (IDA, IGI) promoter clearance from RNA polymerase II promoter (IC) regulation of protein serine/threonine phosphatase activity (IDA) RNA polymerase II transcriptional preinitiation complex assembly (IC) transcription elongation from RNA polymerase II promoter (IDA, IMP) transcription initiation from RNA polymerase II promoter (IDA, IMP) transcriptional start site selection at RNA polymerase II promoter (IGI, IMP)
Cellular Component
Manually curated	transcription factor TFIIF complex (IDA)

Classical genetics
conditional	cold sensitivity: increased heat sensitivity: increased
null	inviable
unspecified	resistance to methyl methanesulfonate: decreased
Large-scale survey
null	inviable
reduction of function	competitive fitness: decreased resistance to methyl methanesulfonate: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	180 total interaction(s) for 84 unique genes/features.
Physical Interactions	Affinity Capture-MS: 95 Affinity Capture-RNA: 3 Affinity Capture-Western: 13 Biochemical Activity: 3 Co-crystal Structure: 2 Co-localization: 1 Co-purification: 10 Reconstituted Complex: 3 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 32 Positive Genetic: 3 Synthetic Growth Defect: 5 Synthetic Lethality: 3 Synthetic Rescue: 6
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:867774 to 869981 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2208 867774..869981 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003418