TEF2/YBR118W Summary Help

Standard Name TEF2 1
Systematic Name YBR118W
Feature Type ORF, Verified
Description Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; TEF2-RFP levels increase during replicative aging; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication (1, 2, 3, 4)
Also known as: EF-1 alpha
Name Description Translation Elongation Factor 1
Gene Product Alias eEF1A 5
Chromosomal Location
ChrII:477671 to 479047 | ORF Map | GBrowse
Gbrowse
Genetic position: 61 cM
Gene Ontology Annotations All TEF2 GO evidence and references
  View Computational GO annotations for TEF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 14 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
218 total interaction(s) for 175 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 76
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 10
  • Biochemical Activity: 12
  • Co-crystal Structure: 2
  • Co-fractionation: 4
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 7
  • Negative Genetic: 54
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 5
  • Positive Genetic: 22
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 458
Molecular Weight (Da) 50,032
Isoelectric Point (pI) 9.72
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:477671 to 479047 | ORF Map | GBrowse
SGD ORF map
Genetic position: 61 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1377 477671..479047 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000322
References cited on this page View Complete Literature Guide for TEF2
1) Schirmaier F and Philippsen P  (1984) Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae. EMBO J 3(13):3311-5
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Murthi A, et al.  (2010) Regulation of tRNA Bidirectional Nuclear-Cytoplasmic Trafficking in Saccharomyces cerevisiae. Mol Biol Cell 21(4):639-49
4) Zhang Y, et al.  (2012) Single cell analysis of yeast replicative aging using a new generation of microfluidic device. PLoS One 7(11):e48275
5) Carr-Schmid A, et al.  (1999) Mutations in a GTP-binding motif of eukaryotic elongation factor 1A reduce both translational fidelity and the requirement for nucleotide exchange. J Biol Chem 274(42):30297-302