TDA1/YMR291W Summary Help

Standard Name TDA1 1
Systematic Name YMR291W
Feature Type ORF, Verified
Description Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description Topoisomerase I Damage Affected 1
Chromosomal Location
ChrXIII:852630 to 854390 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TDA1 GO evidence and references
Molecular Function
Manually curated
High-throughput
Biological Process
High-throughput
Cellular Component
High-throughput
Regulators 6 genes
Resources
Large-scale survey
null
overexpression
repressible
Resources
52 total interaction(s) for 47 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 2
  • Biochemical Activity: 6
  • Reconstituted Complex: 11
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 8
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 586
Molecular Weight (Da) 66,219
Isoelectric Point (pI) 6.94
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:852630 to 854390 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1761 852630..854390 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004905
References cited on this page View Complete Literature Guide for TDA1
1) Reid RJ, et al.  (2011) Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage. Genome Res 21(3):477-86
2) Hunter T and Plowman GD  (1997) The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22(1):18-22
3) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Ptacek J, et al.  (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76